Caloric Restriction Normalizes Obesity-Induced Alterations on Regulators of Skeletal Muscle Growth Signaling.

Abstract

The objective of this study was to establish the impact of caloric restriction on high fat diet-induced alterations on regulators of skeletal muscle growth. We hypothesized that caloric restriction would reverse the negative effects of high fat diet-induced obesity on REDD1 and mTOR-related signaling. Following an initial 8 week period of HF diet-induced obesity, caloric restriction (CR ~30 %) was employed while mice continued to consume either a low (LF) or high fat (HF) diet for 8 weeks. Western analysis of skeletal muscle showed that CR reduced (p < 0.05) the obesity-related effects on the lipogenic protein, SREBP1. Likewise, CR reduced (p < 0.05) the obesity-related effects on the hyperactivation of mTORC1 and ERK1/2 signaling to levels comparable to the LF mice. CR also reduced (p < 0.05) obesity-induced expression of negative regulators of growth, REDD1 and cleaved caspase 3. These findings have implications for on the reversibility of dysregulated growth signaling in obese skeletal muscle, using short-term caloric restriction.

• Authors: Cory M Dungan, Ji Li, David L Williamson
• Journal: Lipids (Lipids) Vol. 51 Issue 8 Pg. 905-12 (08 2016) ISSN: 1558-9307 [Electronic] United States
• PMID: 27289530 (Publication Type: Journal Article)

Chemical References

• Ddit4 protein, mouse
• Transcription Factors
• mTOR protein, mouse
• TOR Serine-Threonine Kinases

Topics

• Animals
• Caloric Restriction (methods)
• Diet, High-Fat (adverse effects)
• Male
• Mice
• Muscle Development
• Muscle, Skeletal (growth & development, metabolism)
• Obesity (chemically induced, diet therapy, metabolism)
• Signal Transduction
• TOR Serine-Threonine Kinases (metabolism)
• Transcription Factors (metabolism)