Abstract |
Cerebral infarction (CI) and intracerebral hemorrhage are lethal cerebrovascular diseases, sometimes sharing similar clinical manifestations but with distinct therapeutic strategies. Delayed treatment usually resulted in poor prognosis. A timely diagnosis report is highly warranted especially in emergency. One hundred twenty-nine CI patients, 73 intracerebral hemorrhage (ICH) patients, and 98 controls were enrolled in this study. A direct injection mass spectrometry metabolomics approach was adopted using dried blood spot samples. This targeted metabolomics analysis focused on absolute quantitation of 23 amino acids, 26 carnitine/ carnitine esters, and 22 calculated ratios parameters. Compared to the normal control group, CI and ICH showed distinct metabolite changes, respectively. For stroke differentiation, Tyr, C5- OH/C0, Cit, Asn, Pro, Val, Arg/Orn, Leu, and Val/Phe were elevated in the CI group. On the contrary, C5:1, Phe/Tyr, (C0 + C2 + C3 + C16 + C18:1)/ Cit, and Met/Leu were of lower levels in the CI group. Using regression model based on some of the above-mentioned parameters, 79.07% of stroke patients from a new set could be definitely confirmed. This study proved the targeted metabolomics analysis was a promising tool for rapid and timely stroke differentiation.
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Authors | Zhansheng Hu, Zhitu Zhu, Yunfeng Cao, Lixuan Wang, Xiaoyu Sun, Jun Dong, Zhongze Fang, Yanhua Fang, Xiaoxue Xu, Peng Gao, Sun Hongzhi |
Journal | Journal of clinical laboratory analysis
(J Clin Lab Anal)
Vol. 30
Issue 6
Pg. 823-830
(Nov 2016)
ISSN: 1098-2825 [Electronic] United States |
PMID | 27278546
(Publication Type: Journal Article)
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Copyright | © 2016 Wiley Periodicals, Inc. |
Chemical References |
- Amino Acids
- Peptide Fragments
- Carnitine
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Amino Acids
(blood)
- Carnitine
- Case-Control Studies
- Cerebral Hemorrhage
(blood, complications)
- Cerebral Infarction
(blood, complications)
- Chromatography, High Pressure Liquid
- Dried Blood Spot Testing
(methods)
- Female
- Humans
- Male
- Mass Spectrometry
(methods)
- Metabolomics
(methods)
- Middle Aged
- Peptide Fragments
- ROC Curve
- Stroke
(complications, etiology)
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