HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Transcriptional Profiling and miRNA-Target Network Analysis Identify Potential Biomarkers for Efficacy Evaluation of Fuzheng-Huayu Formula-Treated Hepatitis B Caused Liver Cirrhosis.

Abstract
Fuzheng-Huayu (FZHY) formula has been found to have a satisfactory effect on hepatitis B-caused cirrhosis (HBC) treatment. However, the efficacy evaluation of FZHY is often challenging. In this study, a randomized, double-blind and placebo-controlled trial was used to evaluate the therapeutic efficacy of FZHY in HBC treatment. In the trial, 35 medical indexes were detected, and 14 indexes had a statistically-significant difference before compared to after the trial. Importantly, the Child-Pugh score also demonstrated FZHY having therapeutic efficacy. Furthermore, the microRNA (miRNA) profiles of 12 serum samples were detected in FZHY groups, and 112 differential-expressed (DE) miRNAs were determined. Using predicted miRNA targets, 13 kernel miRNAs were identified from the established miRNA-target network. Subsequently, quantitative Real-time Polymerase Chain Reaction (qRT-PCR) was used to validate the expression level of 13 identified miRNAs in the trials. The results showed that nine miRNAs have a statistically-significant difference before compared to after FZHY treatment. By means of a logistic regression model, a miRNA panel with hsa-miR-18a-5p, -326, -1182 and -193b-5p was established, and it can clearly improve the accuracy of the efficacy evaluation of FZHY. This study suggested that the particular miRNAs can act as potential biomarkers and obviously increase the diagnostic accuracy for drug evaluation in HBC treatment progression.
AuthorsQilong Chen, Feizhen Wu, Mei Wang, Shu Dong, Yamin Liu, Yiyu Lu, Yanan Song, Qianmei Zhou, Ping Liu, Yunquan Luo, Shibing Su
JournalInternational journal of molecular sciences (Int J Mol Sci) Vol. 17 Issue 6 (Jun 03 2016) ISSN: 1422-0067 [Electronic] Switzerland
PMID27271613 (Publication Type: Journal Article)
Chemical References
  • Biomarkers
  • Drugs, Chinese Herbal
  • MicroRNAs
  • fuzheng huayu
Topics
  • Biomarkers
  • Cluster Analysis
  • Computational Biology (methods)
  • Drugs, Chinese Herbal (pharmacology, therapeutic use)
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Gene Regulatory Networks
  • Hepatitis B (complications)
  • Humans
  • Liver Cirrhosis (drug therapy, etiology, pathology)
  • MicroRNAs (genetics)
  • RNA Interference
  • ROC Curve
  • Transcriptome
  • Treatment Outcome

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: