A novel rare sugar inhibitor of murine herpes simplex keratitis.

Abstract

PURPOSE:

To determine the therapeutic efficacy of a novel rare sugar, l-psicose, for the treatment of HSV-1 induced herpetic stromal keratitis (HSK) in a mouse eye model.

METHODS:

One rare sugar l-psicose was assayed for HSV-1 inhibition of in vitro virus adsorption. The IC50 and IC90 values of l-psicose were determined using plaque reduction assay (PRA) in CV-1 cell. Female Balb/c mice were corneally infected with HSV-1, strain KOS-GFP; A topical eye drop treatment of l-psicose was started 24 h after infection and continued four times daily for ten consecutive days. The severity of HSK was monitored by slit lamp examination in a masked fashion and Infectious HSV-1 shedding was determined by PRA.

RESULTS:

l-psicose was found to have anti-viral activity in vitro at an IC50 dose of 99.5 mM and an IC90 dose of 160 mM. Topical eye drop treatment with 200 mM l-psicose in PBS solution significantly reduced the severity of HSK compared to the mock treatment group. The in vivo mouse ocular model results of l-psicose therapy correlated with accelerated clearance of virus from eye swabs.

CONCLUSION:

The results suggest that topical treatment with rare sugar l-psicose has efficacy against HSK through inhibition of HSV-1.

• Authors: Syed Muniruzzaman, Megan McIntosh, Ahamed Hossain, Ken Izumori, Partha S Bhattacharjee
• Journal: Journal of pharmacological sciences (J Pharmacol Sci) Vol. 131 Issue 2 Pg. 126-30 (Jun 2016) ISSN: 1347-8648 [Electronic] Japan
• PMID: 27262904 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 The Authors. Production and hosting by Elsevier B.V. All rights reserved.

Chemical References

• Antiviral Agents
• psicose
• Fructose

Topics

• Administration, Topical
• Animals
• Antiviral Agents (administration & dosage, therapeutic use)
• Disease Models, Animal
• Eye (drug effects, virology)
• Female
• Fructose (administration & dosage, therapeutic use)
• Herpesvirus 1, Human
• Keratitis, Herpetic (drug therapy)
• Mice, Inbred BALB C
• Mice, Inbred C57BL