Expression and Function of Group IIE Phospholipase A2 in Mouse Skin.
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| Abstract |
Recent studies using knock-out mice for various secreted phospholipase A2 (sPLA2) isoforms have revealed their non-redundant roles in diverse biological events. In the skin, group IIF sPLA2 (sPLA2-IIF), an "epidermal sPLA2" expressed in the suprabasal keratinocytes, plays a fundamental role in epidermal-hyperplasic diseases such as psoriasis and skin cancer. In this study, we found that group IIE sPLA2 (sPLA2-IIE) was expressed abundantly in hair follicles and to a lesser extent in basal epidermal keratinocytes in mouse skin. Mice lacking sPLA2-IIE exhibited skin abnormalities distinct from those in mice lacking sPLA2-IIF, with perturbation of hair follicle ultrastructure, modest changes in the steady-state expression of a subset of skin genes, and no changes in the features of psoriasis or contact dermatitis. Lipidomics analysis revealed that sPLA2-IIE and -IIF were coupled with distinct lipid pathways in the skin. Overall, two skin sPLA2s, hair follicular sPLA2-IIE and epidermal sPLA2-IIF, play non-redundant roles in distinct compartments of mouse skin, underscoring the functional diversity of multiple sPLA2s in the coordinated regulation of skin homeostasis and diseases.
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| Authors | Kei Yamamoto, Yoshimi Miki, Hiroyasu Sato, Yasumasa Nishito, Michael H Gelb, Yoshitaka Taketomi, Makoto Murakami |
| Journal | The Journal of biological chemistry (J Biol Chem) Vol. 291 Issue 30 Pg. 15602-13 (07 22 2016) ISSN: 1083-351X [Electronic] United States |
| PMID | 27226633 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | © 2016 by The American Society for Biochemistry and Molecular Biology, Inc. |
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