Ginsenoside-Rh2 Inhibits Proliferation and Induces Apoptosis of Human Gastric Cancer SGC-7901 Side Population Cells.

Abstract

OBJECTIVES:

To observed the effects of ginsenoside -Rh2 (GS-Rh2) on proliferation and apoptosis of side population (SP) human gastric cancer SGC-7901 cells.

MATERIALS AND METHODS:

SGC-7901 SP and Non-SP cells were sorted by flow cytometry and assessed using the cck-8 method. Expression of apoptosis-related proteins Bax and Bcl-2 of SP before and after the intervention was determined by Western-blotting.

RESULTS:

It was found that the proliferation of SP was significantly faster than that of NSP (<0.05). In addition, GS-Rh2 inhibited proliferation of gastric cancer SP cells, induced cell cycle arrest and cell apoptosis, and changed the expression of BAX/Bcl-2 proteins in a time-dependent and concentration-dependent manner (<0.05).

CONCLUSIONS:

With increase of GS-Rh2 dose, GS-Rh2 gradually inhibit the proliferation of SGC-7901 SP cells, which have high proliferation rate, through G1/G0 phase arrest, followed by apoptosis which involves the up-regulation of Bax and the down-regulation of Bcl-2.

• Authors: Jun Qian, Jing Li, Jian-Guang Jia, Xin Jin, Da-Jun Yu, Chen-Xu Guo, Bo Xie, Li-Yu Qian
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 17 Issue 4 Pg. 1817-21 ( 2016) ISSN: 2476-762X [Electronic] Thailand
• PMID: 27221858 (Publication Type: Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Ginsenosides
• ginsenoside Rh2

Topics

• Apoptosis (drug effects)
• Blotting, Western
• Cell Cycle (drug effects)
• Cell Proliferation (drug effects)
• Drugs, Chinese Herbal (chemistry)
• Flow Cytometry
• Ginsenosides (pharmacology)
• Humans
• Side-Population Cells (drug effects, pathology)
• Stomach Neoplasms (drug therapy, pathology)
• Tumor Cells, Cultured