Abstract | OBJECTIVE: METHODS: RESULTS: GAPDH was identified as a novel autoantigen associated with neuropsychiatric disorders. Serum anti-GAPDH IgG was detected in the serum of 51% of patients with schizophrenia and 50% of patients with major depression. Moreover, SLE patients with comorbid psychiatric manifestations presented significantly higher serum levels of anti-GAPDH antibodies than did SLE patients without psychiatric manifestations (P = 0.004 by chi-square test). Of note, a significant positive correlation (R = 0.48, P = 0.0049, by Spearman's rank correlation test) was found between the levels of serum anti-GAPDH antibodies and cognitive dysfunction in patients with SLE. In vitro analysis of the effects of purified human anti-GAPDH autoantibodies on SH-SY5Y cells showed an immediate neurite retraction. Finally, in vivo administration of anti-GAPDH autoantibodies in the right cerebral ventricle of C57BL/6J mice resulted in specific behavioral changes associated with a detrimental cognitive and emotional profile. CONCLUSION: Overall, these data suggest that anti-GAPDH autoantibodies play a role in the pathogenesis of neuropsychiatric disorders, thus representing a potentially promising tool for the screening of individual vulnerability to these disabling conditions.
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Authors | Federica Delunardo, Denise Soldati, Veronica Bellisario, Alessandra Berry, Serena Camerini, Marco Crescenzi, Cristiano Alessandri, Fabrizio Conti, Fulvia Ceccarelli, Ada Francia, Guido Valesini, Francesca Cirulli, Alberto Siracusano, Alessandra Siracusano, Cinzia Niolu, Ivo Alex Rubino, Elena Ortona, Paola Margutti |
Journal | Arthritis & rheumatology (Hoboken, N.J.)
(Arthritis Rheumatol)
Vol. 68
Issue 11
Pg. 2708-2716
(11 2016)
ISSN: 2326-5205 [Electronic] United States |
PMID | 27213890
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2016, American College of Rheumatology. |
Chemical References |
- Autoantibodies
- Autoantigens
- Biomarkers
- Immunoglobulin G
- Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
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Topics |
- Adult
- Animals
- Autoantibodies
(immunology, pharmacology)
- Autoantigens
- Behavior, Animal
(drug effects)
- Biomarkers
- Bipolar Disorder
(immunology)
- Cell Line, Tumor
- Cognition
(drug effects)
- Cognitive Dysfunction
(immunology)
- Depressive Disorder, Major
(immunology)
- Emotions
(drug effects)
- Female
- Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
(immunology)
- Humans
- Immunoglobulin G
(immunology)
- Injections, Intraventricular
- Lupus Erythematosus, Systemic
(immunology)
- Lupus Vasculitis, Central Nervous System
(immunology)
- Male
- Mice, Inbred C57BL
- Middle Aged
- Neurites
(drug effects)
- Schizophrenia
(immunology)
- Young Adult
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