Effects of zinc oxide nanoparticles on human coronary artery endothelial cells.

Abstract

Inhalation of zinc oxide (ZnO) metal fumes is known to cause metal fume fever and to have systemic effects; however, the effects of ZnO nanoparticles (ZnONPs) on the cardiovascular system remain unclear. The objective of this study was to investigate the cardiovascular toxicity of ZnONPs. Human coronary artery endothelial cells (HCAECs) were exposed to ZnONPs of different sizes to investigate the cell viability, 8-hydroxy-2'-deoxyguanosine (8-OHdG), interleukin (IL)-6, nitric oxide (NO), and regulation of cardiovascular disease-related genes. Exposure of HCAECs to ZnONPs resulted in decreased cell viability and increased levels of 8-OHdG, IL-6, and NO. Downregulation of cardiovascular-associated genes was observed in response to ZnONPs in HCAECs determined by qPCR, suggesting that the calcium signaling pathway, neuroactive ligand-receptor interaction, hypertrophic cardiomyopathy, dilated cardiomyopathy, and renin-angiotensin system are important affected pathways in response to ZnONPs. Furthermore, we observed a significant response of AGTR1 to ZnONP exposure in HCAECs. Our results suggest that ZnONPs cause toxicity to HCAECs, which could be associated with cardiovascular dysfunction.

• Authors: Kai-Jen Chuang, Kang-Yun Lee, Chih-Hong Pan, Ching-Huang Lai, Lian-Yu Lin, Shu-Chuan Ho, Kin-Fai Ho, Hsiao-Chi Chuang
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 93 Pg. 138-44 (Jul 2016) ISSN: 1873-6351 [Electronic] England
• PMID: 27185063 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Ltd. All rights reserved.

Chemical References

• AGTR1 protein, human
• Biomarkers
• Dermatologic Agents
• IL6 protein, human
• Interleukin-6
• RNA, Messenger
• Receptor, Angiotensin, Type 1
• Nitric Oxide
• Zinc Oxide
• Calcium

Topics

• Apoptosis (drug effects)
• Biomarkers (analysis)
• Calcium (metabolism)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Coronary Vessels (drug effects, metabolism, pathology)
• Dermatologic Agents (toxicity)
• Endothelium, Vascular (drug effects, metabolism, pathology)
• Humans
• Interleukin-6 (genetics, metabolism)
• Metal Nanoparticles (toxicity)
• Nitric Oxide (metabolism)
• RNA, Messenger (genetics)
• Real-Time Polymerase Chain Reaction
• Receptor, Angiotensin, Type 1 (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Zinc Oxide (toxicity)