PMK-S005 Alleviates Age-Related Gastric Acid Secretion, Inflammation, and Oxidative Status in the Rat Stomach.

Abstract

BACKGROUND/AIMS:

The aim of this study was to evaluate the effect of the synthetic S-allyl-L-cysteine (SAC) PMK-S005 on gastric acid secretion, inflammation, and antioxidant enzymes in aging rats.

METHODS:

The rats were divided into four groups at 31 weeks of age and were continuously fed a diet containing a vehicle control, PMK-S005 (5 or 10 mg/kg), or lansoprazole (5 mg/kg). Gastric acid secretion and connective tissue thickness of the lamina propria were evaluated at 74 weeks and 2 years of age. Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and COX-2 levels were measured by using enzyme-linked immunosorbent assays (ELISAs) or Western blot assays. Levels of antioxidant enzymes, including heme oxyganase 1 (HO-1) and

NAD(P)H:

quinone oxidoreductase 1 (NQO-1), were also measured.

RESULTS:

As the rats aged, gastric acid secretion significantly decreased, and the connective tissue of the lamina propria increased. However, 74-week-old rats in the PMK-S005 group exhibited greater levels of gastric acid secretion than those of the control and lansoprazole groups. The increase of TNF-α, IL-1β, and COX- 2 expression in 74-week and 2-year-old control rats were inhibited by PMK-S005. In addition, the decrease in HO-1 and NQO-1 protein expression that occurred with aging was inhibited by PMK-S005 in the 74-week-old rats.

CONCLUSIONS:

These results suggest that PMK-S005 has therapeutic potential as an antiaging agent to ameliorate age-related gastric acid secretion, inflammation, and oxidative stress in the stomach.

• Authors: Yoon Jeong Choi, Nayoung Kim, Ju Yup Lee, Ryoung Hee Nam, Ji Hyung Suh, Sun Min Lee, Min Hee Ham, Hyun Jin Jo, Young Kwang Shim, Yo Han Park, Jong-Chan Lee, Yoon Jin Choi, Hye Seung Lee, Dong Ho Lee
• Journal: Gut and liver (Gut Liver) Vol. 10 Issue 5 Pg. 749-56 (Sep 15 2016) ISSN: 2005-1212 [Electronic] Korea (South)
• PMID: 27172930 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Interleukin-1beta
• PMK-S005
• Plant Extracts
• Tumor Necrosis Factor-alpha
• Cyclooxygenase 2

Topics

• Aging (drug effects)
• Animals
• Antioxidants (analysis)
• Cyclooxygenase 2 (analysis)
• Enzyme-Linked Immunosorbent Assay
• Gastric Acid (metabolism)
• Gastric Mucosa (metabolism)
• Gastritis (drug therapy, enzymology)
• Interleukin-1beta (analysis)
• Male
• Oxidative Stress (drug effects)
• Plant Extracts (pharmacology)
• Rats
• Rats, Inbred F344
• Stomach (drug effects, enzymology)
• Tumor Necrosis Factor-alpha (analysis)