Abstract | BACKGROUND: Recently, many lncRNAs were found to be deregulated in various human cancers and play important roles in carcinogenesis. MATERIAL AND METHODS: RESULTS: We have identified 23,169 candidate novel lncRNA transcripts. Comparing with protein coding genes the lncRNAs tend to be shorter and have less exons. Remarkably, we found 15 lncRNAs that were down-regulated in all cancer cell lines among all four types of cancers. In addition, we analyzed the differentially expressed lncRNAs in gastrointestinal cancer cells before and after treatment with 5-Aza. Many lncRNAs were up-regulated after 5-Aza treatment, which suggested that the expression of these lncRNAs may be regulated by DNA methylation. Finally, based on the co-expression network and GO enrichment analysis, we predicted that many novel lncRNAs were involved in pathways like apoptosis, cell cycle, cell adhesion, EMT, epigenetic regulation, DNA damage response signaling, and immune response. CONCLUSION: Based on RNA-Seq and bioinformatics analysis, we have identified a significant number of novel lncRNAs, which could be involved in important pathways related to gastrointestinal cancer development. Overall, we provide a rich resource for identifying new biomarkers and studying novel lncRNA regulation pathways in gastrointestinal cancer.
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Authors | Baoping Cao, Nanxi Song, Meiying Zhang, Chao Di, Yang Yang, Youyong Lu, Runsheng Chen, Zhi John Lu, Mingzhou Guo |
Journal | Discovery medicine
(Discov Med)
Vol. 21
Issue 115
Pg. 159-71
(03 2016)
ISSN: 1944-7930 [Electronic] United States |
PMID | 27115166
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- RNA, Long Noncoding
- Azacitidine
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Topics |
- Apoptosis
(genetics)
- Azacitidine
(pharmacology)
- Biomarkers, Tumor
(genetics)
- Carcinogenesis
(genetics)
- Cell Cycle
(genetics)
- Cell Line, Tumor
- DNA Methylation
- Down-Regulation
- Epigenesis, Genetic
- Epithelial-Mesenchymal Transition
(genetics)
- Gastrointestinal Neoplasms
(genetics)
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Humans
- RNA, Long Noncoding
(drug effects, genetics)
- Real-Time Polymerase Chain Reaction
- Sequence Analysis, RNA
- Signal Transduction
(genetics)
- Up-Regulation
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