Abstract |
Salmonella Gallinarum biovar Pullorum is the causative agent of pullorum disease in poultry, an acute systemic disease that results in a high mortality rate in young chickens. Vaccines have been considered in many developing countries where levels of infection are high and eradication is not a realistic option. An attenuated strain combined with protein E-mediated cell lysis was used to generate a safety enhanced Salmonella Pullorum ghost vaccine. Immune responses and protection induced by ghost vaccine in chickens were investigated following a prime-boost immunization administered via intramuscular and oral routes. Chickens from vaccinated groups showed significant increases in antigen-specific IgG, especially after booster immunization. Lymphocyte proliferation responses were also significantly increased in all immunized groups at 2-weeks post-final vaccination. The Salmonella Pullorum ghost vaccine provided satisfactory protection against virulent Salmonella Pullorum infection, as shown by the robust stimulation of both humoral and cell-mediated immune responses as well as the reduction in the number of bacterial recovered post-challenge. Moreover, the immune effects and survival rates indicated intramuscular injection is more efficient than oral vaccination. In conclusion, our results suggest that Salmonella Pullorum ghosts may be used as a safe and effective novel inactivated vaccine candidate to protect against virulent Salmonella Pullorum infection.
|
Authors | Rongxian Guo, Shizhong Geng, Hongmei Jiao, Zhiming Pan, Xiang Chen, Xinan Jiao |
Journal | Veterinary immunology and immunopathology
(Vet Immunol Immunopathol)
Vol. 173
Pg. 27-33
(May 2016)
ISSN: 1873-2534 [Electronic] Netherlands |
PMID | 27090623
(Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2016 Elsevier B.V. All rights reserved. |
Chemical References |
- E protein, bacteriophage X174
- Salmonella Vaccines
- Vaccines, Inactivated
- Viral Proteins
|
Topics |
- Animals
- Chickens
- Immunity, Cellular
- Poultry Diseases
(immunology, prevention & control)
- Salmonella Infections, Animal
(immunology, pathology, prevention & control)
- Salmonella Vaccines
(administration & dosage, genetics, immunology)
- Salmonella enterica
(immunology)
- Vaccines, Inactivated
(genetics, immunology)
- Viral Proteins
(genetics)
|