N-acetylcysteine leads to greater ovarian protection than enoxaparin sodium in a rat ovarian torsion model.

Abstract

This study evaluated the effects of N-acetylcysteine (NAC) and enoxaparin on ovarian tissue preservation, ovarian reserve and oxidative damage following ovarian torsion/detorsion injury. Rats were divided into four groups (n = 6/group): control; ischaemia/reperfusion (I/R); I/R + NAC; I/R + enoxaparin. Twenty-four hours after detorsion, ovarian tissues were collected for histopathological analysis and measurement of tissue 8-OHdG, GSH, MDA, MPO and SOD concentrations, as well as pre- and post-operative circulating AMH concentrations. Administration of NAC resulted in more pre-antral follicles compared with enoxaparin treatment and haemorrhage and follicle cell degeneration were more pronounced in I/R + enoxaparin group than I/R + NAC group. Both NAC and enoxaparin led to a significant reduction in ovarian tissue 8-OHdG (P = 0.004 and P = 0.01, respectively) and MPO (P = 0.013 and P = 0.023, respectively) concentrations compared with I/R group, indicating a protective effect against I/R oxidative damage. Only NAC-treated animals showed a significant increase in GSH and SOD concentrations and decrease in MDA concentrations compared with I/R group (P = 0.007, P = 0.024 and P = 0.026, respectively). These results indicate that NAC is more effective than enoxaparin in minimizing ovarian damage and preserving ovarian reserve following ovarian torsion.

• Authors: Gulcin Sahin Ersoy, Meryem Eken, Reshef Tal, Deniz Oztekin, Belgin Devranoglu, Ecmel Isik Kaygusuz, Ozge Cevik
• Journal: Reproductive biomedicine online (Reprod Biomed Online) Vol. 33 Issue 1 Pg. 93-101 (Jul 2016) ISSN: 1472-6491 [Electronic] Netherlands
• PMID: 27083693 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Antioxidants
• Enoxaparin
• Malondialdehyde
• Anti-Mullerian Hormone
• 8-Hydroxy-2'-Deoxyguanosine
• Peroxidase
• Superoxide Dismutase
• Deoxyguanosine
• Glutathione
• Acetylcysteine

Topics

• 8-Hydroxy-2'-Deoxyguanosine
• Acetylcysteine (therapeutic use)
• Animals
• Anti-Mullerian Hormone (blood)
• Antioxidants (metabolism)
• Deoxyguanosine (analogs & derivatives, metabolism)
• Enoxaparin (therapeutic use)
• Female
• Glutathione (metabolism)
• In Situ Nick-End Labeling
• Malondialdehyde (metabolism)
• Ovarian Diseases (drug therapy)
• Ovarian Follicle (drug effects)
• Ovary (drug effects)
• Oxidative Stress
• Peroxidase (metabolism)
• Pilot Projects
• Rats
• Rats, Wistar
• Reproductive Techniques, Assisted
• Superoxide Dismutase (metabolism)