As ultrasound diagnosis is applied more frequently in gynecological
acute abdomen, we carried out this study to discuss the diagnosis of
endometriosis with ultrasound imaging and analyze the efficacy of
microRNA used for diagnosing
endometriosis and evaluating prognosis by comparing differentially expressed
microRNA in subjects with or without
endometriosis. Ultrasound examination results and clinical pathological examination results of 60 cases of gynecological
acute abdomen were compared. Blood samples were collected from patients with
endometriosis. Of 60 cases, 38 cases recurred in 20 months after surgery and the remaining 22 cases had no recurrence in the 30-month follow-up. Additionally, blood was collected from 40 patients without
endometriosis as control. Then total
RNA was extracted from these blood samples to determine the difference of expression of
microRNA (miR-17-5p, miR-20a, miR-199a and miR-141). Compared to healthy subjects, the
endometriosis patients showed significantly increased expression of miR-199a, but the expression of miR-17-5p, miR-20a and miR-141 had an obvious decrease; the differences were statistically significant (p less than 0.01). For recurred cases, miR-199 showed a remarkably high expression and miR-17-5p and miR-20a expressed significantly low.