An indirect ELISA for detection of Theileria spp. antibodies using a recombinant protein (rTlSP) from Theileria luwenshuni.

Abstract

Theileria is a tick-borne, intracellular protozoan parasite of worldwide economic and veterinary importance in small ruminants. Here, an enzyme-linked immunosorbent assay (ELISA) was developed based on Theileria luwenshuni recombinant surface protein (rTlSP) and was used in the standardization and validation of an ELISA for the detection of circulating antibodies against ovine and caprine theileriosis. A total of 233 sera samples were used for the calculation of the cut-off value which served as a threshold between the positive and the negative sera. When the positive threshold was chosen as 19% of the specific mean antibody rate, the specificity was 97.9%, and the sensitivity was 97.1%. There was a cross-reaction with sera against Theileria uilenbergi and Theileria ovis, and no cross-reaction with sera against Babesia spp. in the ELISA and Western blotting. Two hundred forty samples collected from sheep in Gansu province were detected with blood smears and ELISA, respectively. The results showed that the positive rate of Theileria infection in Gansu province were 63.75% with rTlSP-ELISA, and 46.67% with blood smears, respectively. Our test proved that the rTlSP ELISA is suitable to diagnose Theileria infection and could be used in serological surveys to map out the prevalence of ovine and caprine theileriosis.

• Authors: Haining He, Youquan Li, Junlong Liu, Zhijie Liu, Jifei Yang, Aihong Liu, Ze Chen, Qiaoyun Ren, Guiquan Guan, Guangyuan Liu, Jianxun Luo, Hong Yin
• Journal: Experimental parasitology (Exp Parasitol) Vol. 166 Pg. 89-93 (Jul 2016) ISSN: 1090-2449 [Electronic] United States
• PMID: 27048941 (Publication Type: Journal Article, Validation Study)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Antibodies, Protozoan
• Membrane Proteins
• Protozoan Proteins
• Recombinant Proteins

Topics

• Animals
• Antibodies, Protozoan (blood)
• Blotting, Western
• China (epidemiology)
• Cross Reactions
• Electrophoresis, Polyacrylamide Gel
• Enzyme-Linked Immunosorbent Assay (methods)
• Goat Diseases (diagnosis, epidemiology, parasitology)
• Goats
• Membrane Proteins (immunology)
• Protozoan Proteins (immunology)
• Recombinant Proteins (immunology)
• Sensitivity and Specificity
• Sheep
• Sheep Diseases (diagnosis, epidemiology, parasitology)
• Theileria (immunology)
• Theileriasis (diagnosis, epidemiology)