Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: We investigated the effects of PA on L-OHP-induced peripheral neuropathies and identified the active ingredient within PA extract. MATERIALS AND METHODS: L-OHP was intraperitoneally injected into mice, and PA boiled water extract was orally administered. Cold and mechanical hyperalgesia were evaluated using the acetone test and the von Frey filament method, respectively. Dorsal root ganglion (DRG) neurons were isolated from normal mice and cultured with L-OHP with or without PA extract. Cell viability and neurite elongation were evaluated. RESULTS: PA extract significantly attenuated cold and mechanical hyperalgesia induced by L-OHP in mice. In cultured DRG neurons, L-OHP reduced cell viability and neurite elongation in a dose-dependent manner. Treatment with PA extract significantly alleviated the L-OHP-induced reduction of neurite elongation, while the cytotoxicity of L-OHP was not affected. Using activity-guided fractionation, we isolated neoline from PA extract as the active ingredient. Neoline significantly alleviated L-OHP-induced reduction of neurite elongation in cultured DRG neurons in a concentration-dependent manner. Moreover, subcutaneous injection of neoline attenuated cold and mechanical hyperalgesia in L-OHP-treated mice. PA extract and neoline did not show sedation and motor impairment. CONCLUSIONS: The present study indicates that PA and its active ingredient neoline are promising agents to alleviate L-OHP-induced neuropathic pain.
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Authors | Toshiaki Suzuki, Keisuke Miyamoto, Naomi Yokoyama, Mayuko Sugi, Akina Kagioka, Yuka Kitao, Takumi Adachi, Masahiro Ohsawa, Hajime Mizukami, Toshiaki Makino |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 186
Pg. 44-52
(Jun 20 2016)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 27038579
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Analgesics
- Organoplatinum Compounds
- neoline
- Oxaliplatin
- Aconitine
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Topics |
- Aconitine
(analogs & derivatives, chemistry, pharmacology)
- Aconitum
(chemistry)
- Analgesics
(chemistry, pharmacology)
- Animals
- Hyperalgesia
(chemically induced, drug therapy)
- Male
- Mice
- Molecular Structure
- Organoplatinum Compounds
(toxicity)
- Oxaliplatin
- Peripheral Nervous System Diseases
(chemically induced, drug therapy)
- Phytotherapy
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