Optimal sequence of antisense DNA to silence YB-1 in lung cancer by use of a novel polysaccharide drug delivery system.

Abstract

Silencing Y-box binding protein 1 (YB-1) can be an excellent target for cancer therapy and many lung cancer cells express the polysaccharide-recognition receptor Dectin-1. We designed a Dectin-1 targeting vehicle delivering YB-1-antisense DNA. First, we selected five optimal antisense DNA sequences to silence YB-1 from among 153 candidates. We chose the sequence closest to the start codon (AS014), and attached dA40 to the 3' end; dA40 promotes complex formation with a β-(1➝3)-d-glucan called schizophyllan (SPG). The resultant complexes were applied to 12 human-oriented lung cancer cell lines, and cell viability was examined. The cell lines exhibited decreased viability and showed strong affinity to bind SPG, suggesting the AS014/SPG complex entered the cells via the Dectin-1 mediated pathway.

• Authors: Hiroto Izumi, Shohei Nagao, Shinichi Mochizuki, Nobuaki Fujiwara, Kazuo Sakurai, Yasuo Morimoto
• Journal: International journal of oncology (Int J Oncol) Vol. 48 Issue 6 Pg. 2472-8 (Jun 2016) ISSN: 1791-2423 [Electronic] Greece
• PMID: 27035516 (Publication Type: Journal Article)

Chemical References

• DNA, Antisense
• Lectins, C-Type
• Y-Box-Binding Protein 1
• YBX1 protein, human
• dectin 1
• Sizofiran

Topics

• Base Sequence
• Cell Line
• Cell Survival (drug effects)
• DNA, Antisense (chemistry, genetics, pharmacology)
• Drug Delivery Systems
• Gene Silencing
• Humans
• Lectins, C-Type (chemistry)
• Lung Neoplasms (genetics)
• Sizofiran (chemistry)
• Y-Box-Binding Protein 1 (antagonists & inhibitors, genetics)