Abstract | SCOPE: Syzygium cumini (jamun) is perhaps the only berry that has the diversity of anthocyanidins of blueberry and bilberry and the abundance of ellagitannins/ ellagic acid of black raspberry. Here, we report the potential of jamun against 17β-estrogen-mediated breast cancer and the role of miRNAs and other targets in disease inhibition. METHODS AND RESULTS: Female August-Copenhagen Irish rats were given AIN-93M diet or diet supplemented with jamun. Two weeks later, animals received 17β-estradiol and were palpated weekly for the mammary tumors. At the end of 26 weeks, the jamun-diet significantly delayed the first tumor appearance by 21 days, and reduced the tumor incidence (65% versus 96%), tumor burden (313 ± 95 versus 661 ± 123 mm(3) ) and tumor multiplicity (1.8 ± 0.3 versus 4.2 ± 0.4 tumors/rat) compared to control. The experimental diet significantly reduced the estrogen-associated growth of pituitary prolactinomas, circulating prolactin and estradiol levels and offset estrogen-associated increases in mammary cell-proliferation, estrogen receptor-alpha (ER-α), and cyclinD1. miRNAs that were either overexpressed (miR-182 and miR-375) or underexpressed (miR-127 and miR-206) following estrogen-treatment were significantly protected by jamun diet. CONCLUSIONS: Together, our data show that jamun significantly offset estrogen-mediated alterations in mammary cell-proliferation, ER-α, cyclinD1, and candidate miRNAs, and that the modulation of these biomarkers correlated with a reduction in mammary carcinogenicity.
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Authors | Farrukh Aqil, Jeyaprakash Jeyabalan, Radha Munagala, Inder Pal Singh, Ramesh C Gupta |
Journal | Molecular nutrition & food research
(Mol Nutr Food Res)
Vol. 60
Issue 6
Pg. 1470-81
(06 2016)
ISSN: 1613-4133 [Electronic] Germany |
PMID | 27030099
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Chemical References |
- Anthocyanins
- Ccnd1 protein, rat
- Estrogen Receptor alpha
- Hydrolyzable Tannins
- MIRN182 microRNA, rat
- MIRN375 microRNA, rat
- MicroRNAs
- Mirn127 microRNA, rat
- Plant Preparations
- mirn206 microRNA, rat
- Cyclin D1
- Ellagic Acid
- Estradiol
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Topics |
- Animals
- Anthocyanins
(pharmacology)
- Breast Neoplasms
(genetics, prevention & control)
- Cell Proliferation
(drug effects)
- Cyclin D1
(genetics, metabolism)
- Diet
- Ellagic Acid
(pharmacology)
- Estradiol
(toxicity)
- Estrogen Receptor alpha
(genetics, metabolism)
- Female
- Fruit
(chemistry)
- Gene Expression Regulation, Neoplastic
- Hydrolyzable Tannins
(pharmacology)
- Mammary Neoplasms, Experimental
- MicroRNAs
(genetics, metabolism)
- Plant Preparations
(pharmacology)
- Rats
- Rats, Inbred Strains
- Syzygium
(chemistry)
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