Efficient Purification and Release of Circulating Tumor Cells by Synergistic Effect of Biomarker and SiO2 @Gel-Microbead-Based Size Difference Amplification.

Abstract

Microfluidics-based circulating tumor cell (CTC) isolation is achieved by using gelatin-coated silica microbeads conjugated to CTC-specific antibodies. Bead-binding selectively enlarges target cell size, providing efficient high-purity capture. CTCs captured can be further released non-invasively. This stratagem enables high-performance CTC isolation for subsequent studies.

• Authors: Qinqin Huang, Bo Cai, Bolei Chen, Lang Rao, Zhaobo He, Rongxiang He, Feng Guo, Libo Zhao, Kiran Kumar Kondamareddy, Wei Liu, Shishang Guo, Xing-Zhong Zhao
• Journal: Advanced healthcare materials (Adv Healthc Mater) Vol. 5 Issue 13 Pg. 1554-9 (07 2016) ISSN: 2192-2659 [Electronic] Germany
• PMID: 27028055 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Antibodies, Neoplasm
• Biomarkers, Tumor
• Silicon Dioxide
• Gelatin

Topics

• Antibodies, Neoplasm (chemistry)
• Biomarkers, Tumor (metabolism)
• Cell Separation (instrumentation, methods)
• Cell Size
• Gelatin (chemistry)
• Humans
• Lab-On-A-Chip Devices
• MCF-7 Cells
• Microspheres
• Neoplastic Cells, Circulating (metabolism, pathology)
• Silicon Dioxide (chemistry)