Feasibility of using DNA-immobilized nanocellulose-based immunoadsorbent for systemic lupus erythematosus plasmapheresis.

Abstract

The goal of this project was to study the feasibility of using a DNA-immobilized nanocellulose-based immunoadsorbent for possible application in medical apheresis such as systemic lupus erythematosus (SLE) treatment. Calf thymus DNA was bound to high surface area nanocellulose membrane at varying concentrations using UV-irradiation. The DNA-immobilized samples were characterized with scanning electron microscopy, atomic force microscopy, and phosphorus elemental analysis. The anti-ds-DNA IgG binding was tested in vitro using ELISA. The produced sample showed high affinity in vitro to bind anti-ds-DNA-antibodies from mice, as much as 80% of added IgG was bound by the membrane. Furthermore, the binding efficiency was quantitatively dependent on the amount of immobilized DNA onto nanocellulose membrane. The described nanocellulose membranes are interesting immunoadsorbents for continued clinical studies.

• Authors: Changgang Xu, Daniel O Carlsson, Albert Mihranyan
• Journal: Colloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 143 Pg. 1-6 (Jul 01 2016) ISSN: 1873-4367 [Electronic] Netherlands
• PMID: 27011345 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies, Antinuclear
• Immobilized Nucleic Acids
• Immunoglobulin G
• Immunosorbents
• Cellulose
• DNA
• calf thymus DNA

Topics

• Animals
• Antibodies, Antinuclear (isolation & purification)
• Cellulose (chemistry, radiation effects)
• DNA (chemistry)
• Disease Models, Animal
• Enzyme-Linked Immunosorbent Assay
• Humans
• Immobilized Nucleic Acids (chemistry)
• Immunoglobulin G (isolation & purification)
• Immunosorbents (chemistry, radiation effects)
• Lupus Erythematosus, Systemic (immunology, pathology, therapy)
• Mice
• Nanostructures (chemistry)
• Plasmapheresis (methods)
• Ultraviolet Rays