Plasma High-Mannose and Complex/Hybrid N-Glycans Are Associated with Hypercholesterolemia in Humans and Rabbits.
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| Abstract |
N-glycans play important roles in various pathophysiological processes and can be used as clinical diagnosis markers. However, plasma N-glycans change and their pathophysiological significance in the setting of hypercholesterolemia, a major risk factor for atherosclerosis, is unknown. Here, we collected plasma from both hypercholesterolemic patients and cholesterol-fed hypercholesterolemic rabbits, and determined the changes in the whole-plasma N-glycan profile by electrospray ionization mass spectrometry. We found that both the hypercholesterolemic patients and rabbits showed a dramatic change in their plasma glycan profile. Compared with healthy subjects, the hypercholesterolemic patients exhibited higher plasma levels of a cluster of high-mannose and complex/hybrid N-glycans (mainly including undecorated or sialylated glycans), whereas only a few fucosylated or fucosylated and sialylated N-glycans were increased. Additionally, cholesterol-fed hypercholesterolemic rabbits also displayed increased plasma levels of high-mannose in addition to high complex/hybrid N-glycan levels. The whole-plasma glycan profiles revealed that the plasma N-glycan levels were correlated with the plasma cholesterol levels, implying that N-glycans may be a target for treatment of hypercholesterolemia.
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| Authors | Liang Bai, Qianwei Li, Lingmei Li, Yan Lin, Sihai Zhao, Weirong Wang, Rong Wang, Yongqin Li, Jiangbei Yuan, Chengjian Wang, Zhongfu Wang, Jianglin Fan, Enqi Liu |
| Journal | PloS one (PLoS One) Vol. 11 Issue 3 Pg. e0146982 ( 2016) ISSN: 1932-6203 [Electronic] United States |
| PMID | 26999365 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Chemical References |
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