Abstract |
Cancer cells in hypoxia usually make adaptive changes in cellular metabolism, such as altered autophagy. This might be a cause of enhanced radioresistance in some types of cancer. In this study, we investigated hypoxia-responsive miRNAs in two prostate cancer cell lines (DU145 and PC3). This study firstly reported that hypoxia induces further downregulation of miR-124 and miR-144, which might be a result of impaired dicer expression. These two miRNAs can simultaneously target 3'UTR of PIM1. Functional study showed that miR-124 or miR-144 overexpression can inhibit hypoxia-induced autophagy and enhance radiosensitivity at least via downregulating PIM1. Therefore, hypoxia induced miR-124 and miR-144 downregulation may contribute to a prosurvival mechanism of prostate cancer cells to hypoxia and irradiation at least through attenuated suppressing of PIM1. This finding presents a potential therapeutic target for prostate cancer.
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Authors | Hao Gu, Mingzhu Liu, Changmao Ding, Xin Wang, Rui Wang, Xinyu Wu, Ruitai Fan |
Journal | Cancer medicine
(Cancer Med)
Vol. 5
Issue 6
Pg. 1174-82
(Jun 2016)
ISSN: 2045-7634 [Electronic] United States |
PMID | 26990493
(Publication Type: Journal Article)
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Copyright | © 2016 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. |
Chemical References |
- 3' Untranslated Regions
- MIRN124 microRNA, human
- MIRN144 microRNA, human
- MicroRNAs
- RNA, Messenger
- PIM1 protein, human
- Proto-Oncogene Proteins c-pim-1
- Ribonuclease III
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Topics |
- 3' Untranslated Regions
- Autophagy
(genetics)
- Base Sequence
- Binding Sites
- Cell Line, Tumor
- Cluster Analysis
- Down-Regulation
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Humans
- Hypoxia
(genetics, metabolism)
- Male
- MicroRNAs
(chemistry, genetics)
- Prostatic Neoplasms
(genetics, metabolism)
- Proto-Oncogene Proteins c-pim-1
(chemistry, genetics)
- RNA Interference
- RNA, Messenger
(chemistry, genetics)
- Radiation Tolerance
(genetics)
- Ribonuclease III
(genetics, metabolism)
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