Protective effect of pterostilbene on testicular ischemia/reperfusion injury in rats.

Abstract

PURPOSE:

To evaluate protective effect of pterostilbene against testicular ischemia/reperfusion (I/R) injury, which results in increased formation of oxidative stress, leading to testicular apoptosis and impaired spermatogenesis.

METHODS:

Thirty two pubertal male Sprague-Dawley rats weighing 180-220g were selected and randomly divided into the following four groups: group A (normal control group), group B (sham-operated group), group C (induced I/R injury group), group D (induced I/R injury group receiving pterostilbene treatment). Johnsen's scores and mean seminiferous tubule diameters were evaluated for histopathologic assessment; germinal cell apoptosis was evaluated by the transferase dUTP nick end labeling (TUNEL) assay and immunohistochemistry for caspases. Malondialdehyde (MDA) levels were assessed as an indicator of oxidative stress and total antioxidant capacity (TAC) was measured.

RESULTS:

Germ cell apoptosis and MDA level significantly increased whereas TAC significantly decreased in group C; moreover, abnormal morphology and impaired spermatogenesis were observed in group C. In contrast, treatment with pterostilbene inhibited lipid peroxidation and apoptosis induced by ROS and restored the antioxidant capacity in group D.

CONCLUSIONS:

These results show that treatment with pterostilbene may be a promising therapy for testicular I/R injury.

• Authors: Hyung Joon Kim, Jae-Won Lee, Bo Ram Hwang, Young-Ah Lee, Jong-In Kim, Yoon Ju Cho, Hyun Jhung Jhun, Jin Soo Han
• Journal: Journal of pediatric surgery (J Pediatr Surg) Vol. 51 Issue 7 Pg. 1192-6 (Jul 2016) ISSN: 1531-5037 [Electronic] United States
• PMID: 26976774 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Antioxidants
• Biomarkers
• Stilbenes
• pterostilbene
• Malondialdehyde

Topics

• Animals
• Antioxidants (pharmacology, therapeutic use)
• Apoptosis (drug effects)
• Biomarkers (metabolism)
• Lipid Peroxidation (drug effects)
• Male
• Malondialdehyde (metabolism)
• Oxidative Stress (drug effects)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, pathology, prevention & control)
• Spermatic Cord Torsion (complications, metabolism, pathology)
• Stilbenes (pharmacology, therapeutic use)
• Testis (drug effects, metabolism, pathology)
• Treatment Outcome