Bioengineered Magnetoferritin Nanoprobes for Single-Dose Nuclear-Magnetic Resonance Tumor Imaging.

Abstract

Despite all the advances in multimodal imaging, it remains a significant challenge to acquire both magnetic resonance and nuclear imaging in a single dose because of the enormous difference in sensitivity. Indeed, nuclear imaging is almost 10(6)-fold more sensitive than magnetic resonance imaging (MRI); thus, repeated injections are generally required to obtain sufficient MR signals after nuclear imaging. Here, we show that strategically engineered magnetoferritin nanoprobes can image tumors with high sensitivity and specificity using SPECT and MRI in living mice after a single intravenous injection. The magnetoferritin nanoprobes composed of (125)I radionuclide-conjugated human H-ferritin iron nanocages ((125)I-M-HFn) internalize robustly into cancer cells via a novel tumor-specific HFn-TfR1 pathway. In particular, the endocytic recycling characteristic of TfR1 transporters solves the nuclear signal blocking issue caused by the high dose nanoprobes injected for MRI, thus enabling simultaneous functional and morphological tumor imaging without reliance on multi-injections.

• Authors: Yanzhao Zhao, Minmin Liang, Xiao Li, Kelong Fan, Jie Xiao, Yanli Li, Hongcheng Shi, Fei Wang, Hak Soo Choi, Dengfeng Cheng, Xiyun Yan
• Journal: ACS nano (ACS Nano) Vol. 10 Issue 4 Pg. 4184-91 (04 26 2016) ISSN: 1936-086X [Electronic] United States
• PMID: 26959856 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• CD71 antigen
• Contrast Media
• Iodine Radioisotopes
• Magnetite Nanoparticles
• Oxides
• Radiopharmaceuticals
• Receptors, Transferrin
• magnetoferritin
• Apoferritins
• Iron

Topics

• Animals
• Antigens, CD (metabolism)
• Apoferritins (chemistry, metabolism)
• Cell Line, Tumor
• Contrast Media (chemistry)
• Female
• Heterografts
• Humans
• Iodine Radioisotopes
• Iron (chemistry)
• Magnetic Resonance Imaging (methods)
• Magnetic Resonance Spectroscopy (methods)
• Magnetite Nanoparticles (chemistry)
• Mice, Inbred BALB C
• Optical Imaging (methods)
• Oxides (chemistry)
• Radiopharmaceuticals (chemistry)
• Receptors, Transferrin (metabolism)
• Tomography, Emission-Computed, Single-Photon (methods)