Long Noncoding RNA RGMB-AS1 Indicates a Poor Prognosis and Modulates Cell Proliferation, Migration and Invasion in Lung Adenocarcinoma.

Abstract

Lung cancer is the most common cause of cancer-related mortality worldwide. It is a complex disease involving multiple genetic and epigenetic alterations. The development of transcriptomics revealed the important role of long non-coding RNAs (lncRNAs) in lung cancer occurrence and development. Here, microarray analysis of lung adenocarcinoma tissues showed the abnormal expression of lncRNA RGMB-AS1. However, the role of lncRNA RGMB-AS1 in lung adenocarcinoma remains largely unknown. We showed that upregulation of lncRNA RGMB-AS1 was significantly correlated with differentiation, TNM stage, and lymph node metastasis. In lung adenocarcinoma cells, downregulation of lncRNA RGMB-AS1 inhibited cell proliferation, migration, invasion, and caused cell cycle arrest at the G1/G0 phase. In vivo experiments showed that lncRNA RGMB-AS1 downregulation significantly suppressed the growth of lung adenocarcinoma. The expression of lncRNA RGMB-AS1 was inversely correlated with that of repulsive guidance molecule b (RGMB) in lung adenocarcinoma tissues, and UCSC analysis and fluorescence detection assay indicated that lncRNA RGMB-AS1 may be involved in the development of human lung adenocarcinoma by regulating RGMB expression though exon2 of RGMB. In summary, our findings indicate that lncRNA RGMB-AS1 may play an important role in lung adenocarcinoma and may serve as a potential therapeutic target.

• Authors: Ping Li, Guojun Zhang, Juan Li, Rui Yang, Shanshan Chen, Shujun Wu, Furui Zhang, Yong Bai, Huasi Zhao, Yuanyuan Wang, Shaozhi Dun, Xiaonan Chen, Qianqian Sun, Guoqiang Zhao
• Journal: PloS one (PLoS One) Vol. 11 Issue 3 Pg. e0150790 ( 2016) ISSN: 1932-6203 [Electronic] United States
• PMID: 26950071 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Retracted Publication)

Chemical References

• RNA, Long Noncoding

Topics

• Adenocarcinoma (diagnosis, genetics, pathology)
• Adenocarcinoma of Lung
• Animals
• Cell Cycle Checkpoints (genetics)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Down-Regulation
• Exons (genetics)
• Female
• Gene Silencing
• Humans
• Lung Neoplasms (diagnosis, genetics, pathology)
• Male
• Mice
• Mice, Inbred BALB C
• Middle Aged
• Neoplasm Invasiveness
• Prognosis
• RNA, Long Noncoding (genetics)
• Up-Regulation