The Synthesis and Evaluations of the (6 8) Ga-Lissamine Rhodamine B (LRB) as a New Radiotracer for Imaging Tumors by Positron Emission Tomography.

Abstract

PURPOSE:

The aim of this study is to synthesize and evaluate (68)Ga-labeled Lissamine Rhodamine B (LRB) as a new radiotracer for imaging MDA-MB-231 and MCF-7 cells induced tumor mice by positron emission tomography (PET).

METHODS:

Firstly, we performed the radio synthesis and microPET imaging of (68)Ga(DOTA-LRB) in athymic nude mice bearing MDA-MB-231 and MCF-7 human breast cancer xenografts. Additionally, the evaluations of (18)F-fluorodeoxyglucose (FDG), as a glucose metabolism radiotracer for imaging tumors in the same xenografts, have been conducted as a comparison.

RESULTS:

The radiochemical purity of (68)Ga(DOTA-LRB) was >95%. MicroPET dynamic imaging revealed that the uptake of (68)Ga(DOTA-LRB) was mainly in normal organs, such as kidney, heart, liver, and brain and mainly excreted from kidney. The MDA-MB-231 and MCF-7 tumors were not clearly visible in PET images at 5, 15, 30, 40, 50, and 60 min after injection of (68)Ga(DOTA-LRB). The tumor uptake values of (18)F-FDG were 3.79 ± 0.57 and 1.93 ± 0.48%ID/g in MDA-MB-231 and MCF-7 tumor xenografts, respectively.

CONCLUSIONS:

(68)Ga(DOTA-LRB) can be easily synthesized with high radiochemical purity and stability; however, it may be not an ideal PET radiotracer for imaging of MDR-positive tumors.

• Authors: Xuena Li, Yafu Yin, Bulin Du, Na Li, Yaming Li
• Journal: BioMed research international (Biomed Res Int) Vol. 2016 Pg. 8549635 ( 2016) ISSN: 2314-6141 [Electronic] United States
• PMID: 26949707 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Contrast Media
• Radiopharmaceuticals
• Rhodamines
• Fluorodeoxyglucose F18
• lissamine rhodamine B

Topics

• Animals
• Breast Neoplasms (diagnostic imaging, metabolism, pathology)
• Contrast Media (chemical synthesis, pharmacology)
• Fluorodeoxyglucose F18 (pharmacology)
• Humans
• MCF-7 Cells
• Mice
• Positron-Emission Tomography (methods)
• Radiopharmaceuticals (pharmacology)
• Rhodamines (chemical synthesis, pharmacology)
• Tissue Distribution
• Xenograft Model Antitumor Assays