Amplexicaule A exerts anti-tumor effects by inducing apoptosis in human breast cancer.

Abstract

Chemotherapy is the main treatment for patients with breast cancer metastases, but natural alternatives have been receiving attention for their potential as novel anti-tumor reagents. Amplexicaule A (APA) is a flavonoid glucoside isolated from rhizomes of Polygonum amplexicaule D. Don var. sinense Forb (PADF). We found that APA has anti-tumor effects in a breast cancer xenograft mouse model and induces apoptosis in breast cancer cell lines. APA increased levels of cleaved caspase-3,-8,-9 and PARP, which resulted from suppression of MCL-1 and BCL-2 expression in the cells. APA also inactivated the Akt/mTOR pathway in breast cancer cells. Thus, APA exerts a strong anti-tumor effect on breast cancer cells, most likely through induction of apoptosis. Our study is the first to identify this novel anti-tumor compound and provides a new strategy for isolation and separation of single compounds from herbs.

• Authors: Meixian Xiang, Hanwen Su, Guangwen Shu, Dingrong Wan, Feng He, Morgann Loaec, Yali Ding, Jun Li, Sinisa Dovat, Gaungzhong Yang, Chunhua Song
• Journal: Oncotarget (Oncotarget) Vol. 7 Issue 14 Pg. 18521-30 (Apr 05 2016) ISSN: 1949-2553 [Electronic] United States
• PMID: 26943775 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Flavonoids
• Glucosides
• Plant Extracts

Topics

• Animals
• Apoptosis (drug effects)
• Breast Neoplasms (drug therapy, pathology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Disease Models, Animal
• Female
• Flavonoids (pharmacology)
• Glucosides (pharmacology)
• Humans
• MCF-7 Cells
• Mice
• Plant Extracts (pharmacology)
• Polygonum (chemistry)
• Random Allocation
• Xenograft Model Antitumor Assays