Icariside II protects against cerebral ischemia-reperfusion injury in rats via nuclear factor-κB inhibition and peroxisome proliferator-activated receptor up-regulation.

Abstract

Icariside II (IRS) is a metabolite of icariin, which is derived from Herba Epimedii. Although the potential therapeutic effects of icariin on ischemic brain injury were well-investigated; the role of IRS in ischemic stroke is still not addressed clearly. Therefore, the current study aimed to evaluate the effects of IRS on cerebral ischemia-reperfusion injury in rats. The rats were pre-treated by IRS (10 or 30 mg kg(-1), twice a day) for 3 days. After pre-treatment, a MCAO (middle cerebral artery occlusion) for 2 h followed by reperfusion for 24 h was applied on the rats to induce the cerebral ischemia injury model. The neurological deficit scores were assessed at 24 h after reperfusion, then animals were sacrificed, infarct volumes were determined by 2,3,5-triphenyltetrazolium chlorid (TTC) staining and protein expression levels of interleukin-1β (IL-1β), transforming growth factor-β1 (TGF-β1), inhibitory κB (IκB), nuclear factor κB (NF-κB) p65, peroxisome proliferator-activated receptor α (PPARα), and peroxisome proliferator-activated receptor γ (PPARγ) were assayed by using Western blot. IRS pretreatment markedly improved the neurological dysfunction and decreased infarct volume in MCAO rats. In addition, IRS inhibited IL-1β and TGF-β1 protein expression, and resulted in beneficial effects such as inhibition of IκB-α degradation and NF-κB activation induced by MCAO, in a dose-dependent manner. Furthermore, IRS increased the protein expression levels of PPARα and PPARγ in the ischemic brain. In conclusion, pretreatment with IRS protects against cerebral ischemic/reperfusion injury via up-regulation of PPARα and PPARγ and inhibition of NF-κB activation.

• Authors: Yuanyuan Deng, Deqing Xiong, Caixia Yin, Bo Liu, Jingshan Shi, Qihai Gong
• Journal: Neurochemistry international (Neurochem Int) Vol. 96 Pg. 56-61 (06 2016) ISSN: 1872-9754 [Electronic] England
• PMID: 26939761 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Ltd. All rights reserved.

Chemical References

• Drugs, Chinese Herbal
• Flavonoids
• NF-kappa B
• Neuroprotective Agents
• PPAR alpha
• PPAR gamma
• baohuoside I

Topics

• Animals
• Brain Ischemia (metabolism, prevention & control)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Flavonoids (pharmacology, therapeutic use)
• Male
• NF-kappa B (antagonists & inhibitors, metabolism)
• Neuroprotective Agents (pharmacology, therapeutic use)
• PPAR alpha (metabolism)
• PPAR gamma (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, prevention & control)
• Up-Regulation (drug effects, physiology)