Recombinant Lactococcus lactis expressing porcine insulin-like growth factor I ameliorates DSS-induced colitis in mice.

Abstract

BACKGROUND:

Insulin-like growth factor I (IGF-I) is one important family of growth factors, which plays key role in intestinal growth, regeneration, and damage repair. However, the low natural abundance of IGF-I limits its research opportunities and practical application in the fields of medicine and animal husbandry. In this study, a tandem repeat strategy was used to express three copies of the same pIGF-I3 protein in L. lactis. The activity of recombinant pIGF-I3 (rpIGF-I3) was further examined by a mouse model of dextran sulfate sodium (DSS)-induced colitis. In addition, the potential of recombinant L. lactis expressing pIGF-I3 to reduce inflammatory disease was evaluated.

RESULTS:

pIGF-I3 could be expressed in L. lactis by the detection of SDS-PAGE and Western blot. Experimental colitis was induced in BALB/c mice by administration of 5 % DSS in drinking water, and the clinical symptoms were observed in DSS-treated mice. Oral administration of recombinant L. lactis expressing pIGF-I3 improved the colonic architecture, and significantly reduced the increase of colonic damage score (P < 0.05). Furthermore, recombinant L. lactis expressing pIGF-I3 treatment significantly reduced serum DAO activity and colonic MPO level, and elevated colonic occludin level compared to the DSS group (P < 0.05).

CONCLUSIONS:

The pIGF-I3 expressed in L. lactis has good biological activity, and oral administration of recombinant L. lactis expressing pIGF-I3 attenuated the symptoms and development of DSS-induced colitis in mice. These suggested that L. lactis could be a potential host bacterium for production and delivery of IGF-I against intestinal diseases.

• Authors: Shujie Liu, Yongming Li, Bo Deng, Ziwei Xu
• Journal: BMC biotechnology (BMC Biotechnol) Vol. 16 Pg. 25 (Mar 01 2016) ISSN: 1472-6750 [Electronic] England
• PMID: 26932768 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Recombinant Proteins
• Insulin-Like Growth Factor I
• Dextran Sulfate

Topics

• Animals
• Colitis (chemically induced, pathology)
• Colon (drug effects, pathology)
• Dextran Sulfate (adverse effects)
• Disease Models, Animal
• Female
• Insulin-Like Growth Factor I (genetics, metabolism, pharmacology)
• Lactococcus lactis (genetics)
• Mice
• Mice, Inbred BALB C
• Recombinant Proteins (genetics, metabolism, pharmacology)
• Swine