[miR-101 inhibits the proliferation and migration of breast cancer cells via downregulating the expression of DNA methyltransferase 3a].

Abstract

OBJECTIVE:

To investigate the effect of microRNA-101 (miR-101) on the proliferation and migration of breast cancer cells and its possible mechanism.

METHODS:

The expressions of miR-101 and DNA methyltransferase 3a (DNMT3a) in breast cancer tissues, corresponding normal breast tissues, breast cancer cells and normal breast cells were detected by real-time quantitative PCR. The lentiviral vectors containing miR-101 and shRNA-DNMT3a sequences were transfected into MDA-MB-231 cells to regulate the expressions of miR-101 and DNMT3a. The expressions of DNMT3a and E-cadherin were determined by Western blotting. The proliferation and migration abilities of MDA-MB-231 cells were evaluated by MTT assay and wound healing assay, respectively.

RESULTS:

Breast cancer tissues and breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-361, HCC70) showed lower miR-101 expression and higher DNMT3a expression than the adjacent normal breast tissues and normal breast cell line. The overexpression of miR-101 resulted in downregulation of DNMT3a and restoration of E-cadherin. Besides, knockdown of DNMT3a by shRNA increased E-cadherin expression. MTT assay and would healing assay showed that miR-101 overexpression significantly inhibited the proliferation and migration abilities of MDA-MB-231 cells.

CONCLUSION:

miR-101 may inhibit MDA-MB-231 cell proliferation and migration by repressing DNMT3a expression and up-regulating E-cadherin expression.

• Authors: Jian Liu, Yamei Pang, Huangzhen Wang, Yanbo Li, Xin Sun, Fei Xu, Hong Ren, Dapeng Liu
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 32 Issue 3 Pg. 299-303 (Mar 2016) ISSN: 1007-8738 [Print] China
• PMID: 26927545 (Publication Type: Journal Article)

Chemical References

• Cadherins
• DNMT3A protein, human
• MIRN101 microRNA, human
• MicroRNAs
• DNA (Cytosine-5-)-Methyltransferases
• DNA Methyltransferase 3A

Topics

• Blotting, Western
• Breast Neoplasms (genetics, metabolism, pathology)
• Cadherins (genetics, metabolism)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Cell Survival (genetics)
• DNA (Cytosine-5-)-Methyltransferases (genetics, metabolism)
• DNA Methyltransferase 3A
• Down-Regulation
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• MCF-7 Cells
• MicroRNAs (genetics)
• Microscopy, Fluorescence
• RNA Interference
• Reverse Transcriptase Polymerase Chain Reaction