[Increased circulating Ly6Chigh monocyte subsets are correlated with cerebral infarct size in cerebral ischemia/reperfusion mouse models].

Abstract

OBJECTIVE:

To investigate the dynamic changes of monocyte subsets after cerebral ischemia/reperfusion in mice and their correlations with infarct size and neurological defects.

METHODS:

Thirty male C57BL/6 mice were randomly divided into two groups: middle cerebral artery occlusion/reperfusion (MCAO/R) group and Sham group. MCAO/R mouse models were induced via the intraluminal suture technique (so called filament or suture model). The modified Neurological Severity Scores (mNSS) was used to assess neurological function 6, 12 hours, 1, 2, 3 days after MCAO/R. Blood samples were collected 1, 2 and 3 days after surgery to determine the dynamic changes of Ly6C(high) and Ly6C(low) subsets by flow cytometry. Triphenyltetrazolium chloride (TTC) staining and HE staining of the brains were also performed on day 1, 2 and 3. The relationships between the changes of monocyte subsets and the cerebral infarct size and neurological scores were studied by correlation analysis.

RESULTS:

Compared with the baseline, the proportion of Ly6C(high) monocytes significantly increased 1 day after MCAO/R surgery, reached the peak level on the following day and then declined. Compared with the Sham group, the proportion of Ly6C(high) monocytes went up obviously at each time point. TTC staining revealed that the infarct size increased markedly 2 days after surgery. The proportion of Ly6C(high) monocytes in the MCAO/R group was positively associated with the percentage of cerebral infarct size, and it also showed a positive correlation with neurological function deficit scores.

CONCLUSION:

The dynamic changes of monocyte subsets after MCAO/R modeling revealed that Ly6C(high) subset peaked on day 2 after the operation and was correlated with cerebral infarct size.

• Authors: Xin Zhang, Hongxia Li, Yuxiu Li, Yongqiang Ma, Yanwei Luo, Wenjie Ji, Xin Zhou, Yuming Li
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 32 Issue 3 Pg. 294-8 (Mar 2016) ISSN: 1007-8738 [Print] China
• PMID: 26927544 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antigens, Ly
• Ly-6C antigen, mouse

Topics

• Animals
• Antigens, Ly (metabolism)
• Brain (blood supply, metabolism, physiopathology)
• Brain Ischemia (blood, metabolism, physiopathology)
• Cerebral Infarction (blood, metabolism, pathology)
• Disease Models, Animal
• Flow Cytometry
• Infarction, Middle Cerebral Artery (blood, metabolism, physiopathology)
• Male
• Mice, Inbred C57BL
• Monocytes (metabolism)
• Random Allocation
• Reperfusion Injury (blood, metabolism, physiopathology)
• Time Factors