Abstract |
Epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) therapy is an option for lung cancers harboring wild-type EGFR when chemotherapeutic reagents have failed. In this study, we found that the EGFR-TKI, gefitinib, modestly suppressed proliferation of the lung cancer cell lines, A549 and H358, which both harbor wild-type EGFR. Treatment with hepatocyte growth factor (HGF) reduced the sensitivity to gefitinib, whereas sensitivity was restored by treatment with an HGF antibody, a MET inhibitor, or depletion of MET but not ErbB3 gene. Moreover, both PI3K/ mTOR inhibitors and MEK inhibitors suppressed proliferation of A549 cells, whereas only PI3K/ mTOR inhibitors effectively suppressed cell viability of EGFR mutant PC-9 cells. Our findings suggest that HGF reduced the gefitinib sensitivity through MET and downstream PI3K and MAPK pathways. Combined use of EGFR-TKI and MET inhibitors or inhibition of downstream signaling molecules might be a better second or third line choice for a group of patients with advanced lung cancer harboring wild-type EGFR.
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Authors | Hua Yang, Rong Wang, Shunli Peng, Longhua Chen, Qi Li, Wei Wang |
Journal | Oncotarget
(Oncotarget)
Vol. 7
Issue 13
Pg. 16273-81
(Mar 29 2016)
ISSN: 1949-2553 [Electronic] United States |
PMID | 26919104
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- HGF protein, human
- Protein Kinase Inhibitors
- Quinazolines
- Hepatocyte Growth Factor
- ErbB Receptors
- Proto-Oncogene Proteins c-met
- Gefitinib
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Topics |
- Adenocarcinoma
(genetics, metabolism, pathology)
- Adenocarcinoma of Lung
- Antineoplastic Agents
(pharmacology)
- Cell Line, Tumor
- Drug Resistance, Neoplasm
(drug effects, physiology)
- ErbB Receptors
(antagonists & inhibitors)
- Gefitinib
- Hepatocyte Growth Factor
(metabolism, pharmacology)
- Humans
- Lung Neoplasms
(genetics, metabolism, pathology)
- Protein Kinase Inhibitors
(pharmacology)
- Proto-Oncogene Proteins c-met
(antagonists & inhibitors)
- Quinazolines
(pharmacology)
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