IR-780 Loaded Phospholipid Mimicking Homopolymeric Micelles for Near-IR Imaging and Photothermal Therapy of Pancreatic Cancer.

Abstract

IR-780 iodide, a near-infrared (near-IR) fluorescent dye, can be utilized as an effective theranostic agent for both imaging and photothermal therapy. However, its lipophilicity limits its further biomedical applications. Herein, we synthesized a phospholipid mimicking amphiphilic homopolymer poly(12-(methacryloyloxy)dodecyl phosphorylcholine) (PMDPC) via reversible addition-fragmentation chain transfer (RAFT) polymerization. The amphiphilic homopolymer PMDPC can be self-assembled into micelles and used for the encapsulation of IR-780. The IR-780 loaded micelles (PMDPC-IR-780) exhibited low cytotoxicity in the dark, whereas remarkable photothermal cytotoxicity to pancreatic cancer cells (BxPC-3) was observed upon near-IR laser irradiation. We further investigated in vivo biodistribution of PMDPC-IR-780 micelles. Higher accumulation of PMDPC-IR-780 than that of free IR-780 in tumor tissue was verified, which might be ascribed to the enhanced permeability and retention (EPR) effect and long circulation time benefiting from the zwitterionic phosphorylcholine surface. Therefore, the IR-780 loaded phospholipid mimicking homopolymeric micelles could have great potential for cancer theranostics.

• Authors: Yangjun Chen, Zuhong Li, Haibo Wang, Yin Wang, Haijie Han, Qiao Jin, Jian Ji
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 8 Issue 11 Pg. 6852-8 (Mar 23 2016) ISSN: 1944-8252 [Electronic] United States
• PMID: 26918365 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 2-(2-(2-chloro-3-((1,3-dihydro-3,3-dimethyl-1-propyl-2H-indol-2-ylidene)ethylidene)-1-cyclohexen-1-yl)ethenyl)-3,3-dimethyl-1-propylindolium
• Contrast Media
• Indoles
• Micelles
• Phosphorylcholine

Topics

• Animals
• Cell Line, Tumor
• Contrast Media (chemistry, pharmacology)
• Heterografts
• Humans
• Hyperthermia, Induced (methods)
• Indoles (chemistry, pharmacology)
• Male
• Mice, Inbred BALB C
• Mice, Nude
• Micelles
• Neoplasm Transplantation
• Optical Imaging
• Pancreatic Neoplasms (diagnostic imaging, therapy)
• Phosphorylcholine (chemistry, pharmacology)
• Phototherapy (methods)