MicroRNA expression profiling identifies decreased expression of miR-205 in inflammatory breast cancer.

Abstract

Inflammatory breast cancer is the most aggressive form of breast cancer. Identifying new biomarkers to be used as therapeutic targets is in urgent need. Messenger RNA expression profiling studies have indicated that inflammatory breast cancer is a transcriptionally heterogeneous disease, and specific molecular targets for inflammatory breast cancer have not been well established. We performed microRNA expression profiling in inflammatory breast cancer in comparison with locally advanced noninflammatory breast cancer in this study. Although many microRNAs were differentially expressed between normal breast tissue and tumor tissue, most of them did not show differential expression between inflammatory and noninflammatory tumor samples. However, by microarray analysis, quantitative reverse transcription PCR, and in situ hybridization, we showed that microRNA-205 expression was decreased not only in tumor compared with normal breast tissue, but also in inflammatory breast cancer compared with noninflammatory breast cancer. Lower expression of microRNA-205 correlated with worse distant metastasis-free survival and overall survival in our cohort. A small-scale immunohistochemistry analysis showed coexistence of decreased microRNA-205 expression and decreased E-cadherin expression in some ductal tumors. MicroRNA-205 may serve as a therapeutic target in advanced breast cancer including inflammatory breast cancer.

• Authors: Lei Huo, Yan Wang, Yun Gong, Savitri Krishnamurthy, Jing Wang, Lixia Diao, Chang-Gong Liu, Xiuping Liu, Feng Lin, William F Symmans, Wei Wei, Xinna Zhang, Li Sun, Ricardo H Alvarez, Naoto T Ueno, Tamer M Fouad, Kenichi Harano, Bisrat G Debeb, Yun Wu, James Reuben, Massimo Cristofanilli, Zhuang Zuo
• Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (Mod Pathol) Vol. 29 Issue 4 Pg. 330-46 (Apr 2016) ISSN: 1530-0285 [Electronic] United States
• PMID: 26916073 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• MIRN205 microRNA, human
• MicroRNAs

Topics

• Adult
• Aged
• Biomarkers, Tumor (analysis, genetics)
• Female
• Gene Expression Profiling
• Humans
• Immunohistochemistry
• In Situ Hybridization
• Inflammatory Breast Neoplasms (genetics, mortality)
• Kaplan-Meier Estimate
• MicroRNAs (analysis, biosynthesis)
• Middle Aged
• Oligonucleotide Array Sequence Analysis
• Prognosis
• Reverse Transcriptase Polymerase Chain Reaction