Bakuchiol augments MyoD activation leading to enhanced myoblast differentiation.

Abstract

Myoblast differentiation is fundamental to skeletal muscle development and regeneration after injury and defects in this process are implicated in muscle atrophy associated with aging or pathological conditions. MyoD family transcription factors function as mater regulators in induction of muscle-specific genes during myoblast differentiation. We have identified bakuchiol, a prenylated phenolic monoterpene, as an inducer of MyoD-mediated transcription and myogenic differentiation. C2C12 myoblasts treated with bakuchiol exhibit enhanced muscle-specific gene expression and myotube formation. A key promyogenic kinase p38MAPK is activated dramatically by bakuchiol which in turn induced the formation of MyoD/E protein active transcription complexes. Consistently, the recruitment of MyoD and Baf60c to the Myogenin promoter is enhanced in bakuchiol-treated myoblasts. Furthermore, bakuchiol rescues defective p38MAPK activation and myogenic differentiation caused by Cdo-depletion or in RD rhabdomyosarcoma cells. Taken together, these results indicate that bakuchiol enhances myogenic differentiation through p38MAPK and MyoD activation. Thus bakuchiol can be developed into a potential agent to improve muscular regeneration and repair to treat muscular atrophy.

• Authors: Sang-Jin Lee, Miran Yoo, Ga-Yeon Go, Do Hee Kim, Hyunmo Choi, Young-Eun Leem, Yong Kee Kim, Dong-Wan Seo, Jae-Ha Ryu, Jong-Sun Kang, Gyu-Un Bae
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 248 Pg. 60-7 (03 25 2016) ISSN: 1872-7786 [Electronic] Ireland
• PMID: 26902638 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Chromosomal Proteins, Non-Histone
• MyoD Protein
• Phenols
• SMARCD3 protein, human
• Transcription Factors
• p38 Mitogen-Activated Protein Kinases
• bakuchiol

Topics

• Animals
• Cell Differentiation (drug effects, physiology)
• Cell Line
• Chromosomal Proteins, Non-Histone
• Gene Expression Regulation (drug effects, physiology)
• Humans
• Mice
• Molecular Structure
• Muscle Development (drug effects, physiology)
• Muscle, Skeletal (drug effects, physiology)
• Muscular Atrophy (drug therapy)
• MyoD Protein (genetics, metabolism)
• Myoblasts (cytology, drug effects, physiology)
• Phenols (chemistry, pharmacology)
• Regeneration (drug effects, physiology)
• Transcription Factors (metabolism)
• Up-Regulation
• p38 Mitogen-Activated Protein Kinases (genetics, metabolism)