MicroRNA-939 down-regulates CD2-associated protein by targeting promoter in HEK-293T cells.

Abstract

CD2-associated protein (CD2AP) serves as a slit diaphragm (SD) protein and plays essential roles in maintaining podocyte integrity and reducing proteinuria. MicroRNAs (miRNAs) are novel regulators of gene expression. Podocyte-specific loss of miRNAs would lead to significant proteinuria. Here, we report new evidence in which miRNAs may function to suppress CD2AP expression through a transcriptional way. By scanning human CD2AP promoter in silico for sequences complementary to known miRNAs, we chose miR-939, miR-148b*, miR-191*, miR-638 as four candidates and transfected them into HEK-293T cells. Dual-luciferase reporter assay identified that only miR-939 significantly reduced the relative luciferase activity of CD2AP promoter region. Further analysis confirmed that the mRNA and protein expressions of CD2AP were also down-regulated by miR-939. In conclusion, we have identified that miR-939 targets CD2AP promoter sequences and suppresses its gene expression. These findings suggest that miRNAs may mediate podocyte injury via reducing the expression of the SD proteins, such as CD2AP.

• Authors: Yu-Ping Huang, Ling-Zhi Qiu, Guo-Ping Zhou
• Journal: Renal failure (Ren Fail) Vol. 38 Issue 4 Pg. 508-13 ( 2016) ISSN: 1525-6049 [Electronic] England
• PMID: 26895305 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adaptor Proteins, Signal Transducing
• CD2-associated protein
• Cytoskeletal Proteins
• MIRN939 microRNA, human
• MicroRNAs

Topics

• Adaptor Proteins, Signal Transducing (biosynthesis)
• Cells, Cultured
• Cytoskeletal Proteins (biosynthesis)
• Down-Regulation
• Gene Expression Regulation
• HEK293 Cells
• Humans
• MicroRNAs (physiology)
• Podocytes (physiology)
• Promoter Regions, Genetic