MALAT1 long ncRNA promotes gastric cancer metastasis by suppressing PCDH10.

Abstract

EZH2, the catalytic component of polycomb repressive complex 2 (PRC2), is frequently overexpressed in human cancers and contributes to tumor initiation and progression, in part through transcriptional silencing of tumor suppressor genes. A number of noncoding RNAs (ncRNAs) recruit EZH2 to specific chromatin loci, where they modulate gene expression. Here, we used RNA immunoprecipitation sequencing (RIP-seq) to profile EZH2-associated transcripts in human gastric cancer cell lines. We identified 8,256 transcripts, including both noncoding and coding transcripts, some of which were derived from cancer-related loci. In particular, we found that long noncoding RNA (lncRNA) MALAT1 binds EZH2, suppresses the tumor suppressor PCDH10, and promotes gastric cellular migration and invasion. Our work thus provides a global view of the EZH2-associated transcriptome and offers new insight into the function of EZH2 in gastric tumorigenesis.

• Authors: Ying Qi, Hong Sain Ooi, Jun Wu, Jian Chen, Xiaoli Zhang, Sheng Tan, Qing Yu, Yuan-Yuan Li, Yani Kang, Hua Li, Zirui Xiong, Tao Zhu, Bingya Liu, Zhifeng Shao, Xiaodong Zhao
• Journal: Oncotarget (Oncotarget) Vol. 7 Issue 11 Pg. 12693-703 (Mar 15 2016) ISSN: 1949-2553 [Electronic] United States
• PMID: 26871474 (Publication Type: Journal Article)

Chemical References

• Cadherins
• MALAT1 long non-coding RNA, human
• PCDH10 protein, human
• Protocadherins
• RNA, Long Noncoding
• EZH2 protein, human
• Enhancer of Zeste Homolog 2 Protein

Topics

• Cadherins (metabolism)
• Cell Line, Tumor
• Enhancer of Zeste Homolog 2 Protein (metabolism)
• Gene Expression Regulation, Neoplastic (physiology)
• Humans
• Neoplasm Invasiveness (genetics, pathology)
• Protocadherins
• RNA, Long Noncoding (metabolism)
• Stomach Neoplasms (genetics, pathology)