Simultaneous quantification of sulforaphene and sulforaphane by reverse phase HPLC and their content in Raphanus sativus L. var. caudatus Alef extracts.

Abstract

A simple, rapid and precise HPLC assay was developed for the well-known anti-cancer isothiocyanates-sulforaphene (SE) and sulforaphane (SF). The analytical system comprised RP-C18 column with isocratic 5% THF-95% water. High resolution was obtained (and eluted) of two distinct HPLC peaks of similar structures SE and SF analytes (at 23.01±0.02 and 25.65±0.03 min, respectively). The respective LOD vs. LOQ for SE and SF was 0.34 and 0.36 μg/ml vs. 1.02 and 1.08 μg/ml. This assay had the best linearity and accuracy. The recoveries were in the range of 96.83-101.17%. SF and SE were quantified in the pod of Raphanus sativus L. var. caudatus Alef extracts (2253.05±246.18 and 111.94±16.49 μg/g in the crude extract, respectively), while only SE was detected in the stem (1105.14±243.10 μg/g crude extract), as SF was lower than the detection limit. The validated method thus minimized and expedited simultaneous SE and SF analysis.

• Authors: Sarita Sangthong, Natthida Weerapreeyakul
• Journal: Food chemistry (Food Chem) Vol. 201 Pg. 139-44 (Jun 15 2016) ISSN: 1873-7072 [Electronic] England
• PMID: 26868558 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Ltd. All rights reserved.

Chemical References

• Isothiocyanates
• Plant Extracts
• Sulfoxides
• Thiocyanates
• sulforaphane
• sulphoraphene

Topics

• Chromatography, High Pressure Liquid (methods)
• Isothiocyanates (chemistry)
• Plant Extracts (chemistry)
• Raphanus (chemistry)
• Sulfoxides
• Thiocyanates (chemistry)