Prolonged Mouse Cardiac Graft Cold Storage via Attenuating Ischemia-Reperfusion Injury Using a New Antioxidant-Based Preservation Solution.

Abstract

BACKGROUND:

One of the major events in ischemia-reperfusion (I/R)-induced heart injury in cardiac transplantation is the generation of reactive oxygen species. We hypothesized that a novel preservation solution called SBI-SEIIKU II (SS-II) contains 3 antioxidant reagents: L-cysteine, glycine, ascorbic acid/ascorbic acid-2-phosphate magnesium, which can block the generation of reactive oxygen species to result in a prolongation of the cold storage time via attenuating I/R injury.

METHODS:

C57BL/6CrSlc(B6) mice underwent syngeneic mice heterotopic heart transplantation, and the animals were derived into 3 groups: recipients with nonpreserved grafts (control group), recipients with grafts preserved in histidine-tryptophan-ketoglutarate (HTK) for 24 and 48 hours (HTK group), and recipients with grafts preserved in SS-II for 24 and 48 hours (SS-II group).

RESULTS:

After 48 hours of preservation, there were no grafts that survived in the HTK group; however, the SS-II group had a high survival rate. After 24 hours of preservation, SS-II decreased the oxidative damage, myocardial apoptosis, and the infiltration of macrophages and neutrophils in the cardiac grafts in the early phase and suppressed the development of myocardial fibrosis in long-term grafts compared with HTK.

CONCLUSIONS:

The SS-II prolongs the acceptable cold storage time and protects the myocardium from I/R injury via inhibiting oxidative stress-associated damage. We believe that this novel preservation solution may be simple and safe for use in the clinical transplantation field.

• Authors: Songjie Cai, Naotsugu Ichimaru, Mingyi Zhao, Masayuki Fujino, Hidenori Ito, Urara Ota, Motowo Nakajima, Tohru Tanaka, Norio Nonomura, Xiao-Kang Li, Shiro Takahara
• Journal: Transplantation (Transplantation) Vol. 100 Issue 5 Pg. 1032-40 (05 2016) ISSN: 1534-6080 [Electronic] United States
• PMID: 26845308 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Membrane Proteins
• Organ Preservation Solutions
• Organophosphorus Compounds
• Reactive Oxygen Species
• ascorbate-2-polyphosphate
• Heme Oxygenase-1
• Hmox1 protein, mouse
• Magnesium
• Cysteine
• Ascorbic Acid
• Glycine

Topics

• Animals
• Antioxidants (chemistry)
• Apoptosis
• Ascorbic Acid (analogs & derivatives, chemistry)
• Cold Temperature
• Cryopreservation
• Cysteine (chemistry)
• Glycine (chemistry)
• Graft Survival
• Heart (drug effects)
• Heart Injuries (pathology)
• Heart Transplantation (methods)
• Heme Oxygenase-1 (metabolism)
• Macrophages (metabolism)
• Magnesium (chemistry)
• Male
• Membrane Proteins (metabolism)
• Mice
• Mice, Inbred C57BL
• Mitochondria (metabolism)
• Myocardium (metabolism)
• Neutrophils (metabolism)
• Organ Preservation
• Organ Preservation Solutions (chemistry)
• Organophosphorus Compounds (chemistry)
• Oxidative Stress
• Reactive Oxygen Species (metabolism)
• Reperfusion Injury (therapy)
• Time Factors