[MiR-519 inhibits gastric cancer cell activity through regulation of HuR expression].

Abstract

OBJECTIVE:

To investigate the role of miR-519 in regulating the cell activity of gastric cancer. 


METHODS:

The expression of miR-519 was measured by qPCR. The protein expression of HuR was detected by Western blot. MTT assay was used to detect the cell activity of gastric cancer cells.


RESULTS:

The protein level of HuR in the gastric cancer cells was higher than that in the control cells. Over-expression of HuR resulted in the increase in the cell activity of gastric cancer (P<0.001). Suppression of HuR decreased the cell activity of gastric cancer (P=0.001). The miR-519 level in the gastric cancer cells was lower than that in the control cells (P=0.001). Over-expression of miR-519 decreased the protein level of HuR and the activity of gastric cancer cells.


CONCLUSION:

MiR-may inhibit the gastric cancer cell activity through suppression of HuR expression.

• Authors: Ning Zhou, Yang Zhou, Yong Tang, Wenyan Yu
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 41 Issue 1 Pg. 19-23 (Jan 2016) ISSN: 1672-7347 [Print] China
• PMID: 26819420 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ELAV-Like Protein 1
• ELAVL1 protein, human
• MIRN519 microRNA, human
• MicroRNAs

Topics

• Cell Line, Tumor
• Cell Proliferation
• ELAV-Like Protein 1 (genetics, metabolism)
• Gene Expression Regulation, Neoplastic
• Humans
• MicroRNAs (genetics, metabolism)
• Stomach Neoplasms (genetics, metabolism)