Biodegradable nanofiber-membrane for sustainable release of lidocaine at the femoral fracture site as a periosteal block: In vitro and in vivo studies in a rabbit model.

Abstract

The aim of this study was to evaluate the efficacy of a biodegradable, lidocaine-embedded, nanofibrous membrane for the sustainable analgesic release onto fragments of a segmental femoral fracture site. Membranes of three different lidocaine concentrations (10%, 30%, and 50%) were produced via an electrospinning technique. In vitro lidocaine release was assessed by high-performance liquid chromatography. A femoral segmental fracture, with intramedullary Kirschner-wire fixation and polycaprolactone stent enveloping the fracture site, was set-up in a rabbit model for in vivo assessment of post-operative recovery of activity. Eighteen rabbits were randomly assigned to three groups (six rabbits per group): group A comprised of rabbits with femoral fractures and underwent fixation; group B comprised of a comparable fracture model to that of group A with the implantation of lidocaine-loaded nanofibers; and group C, the control group, received only anesthesia. The following variables were measured: change in body weight, food and water intake before and after surgery, and total activity count post-surgery. All membranes eluted effective levels of lidocaine for more than 3 weeks post-surgery. Rabbits in group B showed faster recovery of activity post-operatively, compared with those in group A, which confirmed the pain relief efficacy of the lidocaine-embedded nanofibers. Nanofibers with sustainable lidocaine release have adequate efficacy and durability for pain relief in rabbits with segmental long bone fractures.

• Authors: Ying-Chao Chou, Yi-Shiun Cheng, Yung-Heng Hsu, Yi-Hsun Yu, Shih-Jung Liu
• Journal: Colloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 140 Pg. 332-341 (Apr 01 2016) ISSN: 1873-4367 [Electronic] Netherlands
• PMID: 26774570 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Anesthetics, Local
• Delayed-Action Preparations
• Membranes, Artificial
• Lidocaine

Topics

• Absorbable Implants
• Anesthetics, Local (chemistry, pharmacokinetics, pharmacology)
• Animals
• Body Weight (drug effects)
• Delayed-Action Preparations (chemistry, pharmacokinetics, pharmacology)
• Drinking (drug effects)
• Drug Liberation
• Eating (drug effects)
• Femoral Fractures (physiopathology, surgery)
• Lidocaine (chemistry, pharmacokinetics, pharmacology)
• Membranes, Artificial
• Microscopy, Electron, Scanning
• Nanofibers (chemistry, ultrastructure)
• Nerve Block (methods)
• Pain, Postoperative (prevention & control)
• Periosteum (innervation)
• Rabbits
• Spectroscopy, Fourier Transform Infrared
• Time Factors
• Treatment Outcome