Hepatoprotective effect of trimethylgallic acid esters against carbon tetrachloride-induced liver injury in rats.

Abstract

Gallic acid and its derivatives are potential therapeutic agents for treating various oxidative stress mediated disorders. In the present study, we investigated the hepatoprotective effects of newly synthesized conjugated trimethylgallic acid (TMGA) esters against carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. Animals were pre-treated with TMGA esters at their respective doses for 7 days against CCl4-induced hepatotoxicity. The histopathological changes were evaluated to find out degenerative fatty changes including vacuole formation, inflammation and tissue necrosis. Various biomarkers of oxidative stress (lipid peroxidation, glutathione levels, and endogenous antioxidant enzyme activities), liver enzymes (AST and ALT), triacylglycerol and cholesterol were evaluated. Pre-treatment with TMGA esters (MRG, MGG, MSG, and MUG at the dose of 28.71, 30.03, 31.35, 33.62 mg/kg/day), respectively reversed the CCl4-induced liver injury scores (reduced vacuole formation, inflammation and necrosis), biochemical parameters of plasma (increased AST, ALT, TG, and cholesterol), antioxidant enzymes (increased lipid peroxidation and nitrite levels; decreased glutathione levels, superoxide dismutase and catalase activities) in liver tissues and inflammatory surge (serum TNF-α) significantly. The study revealed that TMGA esters exerted hepatoprotective effects in CCl4-induced rats, specifically by modulating oxidative-nitrosative stress and inflammation.

• Authors: Mamta Sachdeva, Renu Chadha, Anil Kumar, Maninder Karan, Tejvir Singh, Sameer Dhingra
• Journal: Indian journal of experimental biology (Indian J Exp Biol) Vol. 53 Issue 12 Pg. 803-9 (Dec 2015) ISSN: 0019-5189 [Print] India
• PMID: 26742325 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Biomarkers
• Esters
• Triglycerides
• Tumor Necrosis Factor-alpha
• Gallic Acid
• Cholesterol
• Carbon Tetrachloride

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants (pharmacology)
• Biomarkers (blood)
• Carbon Tetrachloride
• Chemical and Drug Induced Liver Injury (blood, etiology, pathology, prevention & control)
• Cholesterol (blood)
• Cytoprotection
• Disease Models, Animal
• Esters (pharmacology)
• Gallic Acid (analogs & derivatives, pharmacology)
• Lipid Peroxidation (drug effects)
• Liver (drug effects, metabolism, pathology)
• Male
• Necrosis
• Oxidative Stress (drug effects)
• Rats, Wistar
• Time Factors
• Triglycerides (blood)
• Tumor Necrosis Factor-alpha (blood)