Abstract | BACKGROUND: METHODS: EAE was induced by immunization with myelin oligodendroglial glycoprotein peptide (MOG)35-55 in C57BL/6 mice. After immunization, mice were observed every 48 hours for signs of EAE and weight loss. At the onset of disease, approximately 14 days after immunization, EAE mice were subjected to a single intravenous injection of hPDLSCs (10(6) cells/150 μl) into the tail vein. At the point of animal sacrifice on day 56 after EAE induction, spinal cord and brain tissues were collected in order to perform histological evaluation, immunohistochemistry and western blotting analysis. RESULTS: CONCLUSIONS: In light of the achieved results, transplantation of hPDLSCs may represent a putative novel and helpful tool for multiple sclerosis treatment. These cells could have considerable implication for future therapies for multiple sclerosis and this study may represent the starting point for further investigations.
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Authors | Oriana Trubiani, Sabrina Giacoppo, Patrizia Ballerini, Francesca Diomede, Adriano Piattelli, Placido Bramanti, Emanuela Mazzon |
Journal | Stem cell research & therapy
(Stem Cell Res Ther)
Vol. 7
Pg. 1
(Jan 04 2016)
ISSN: 1757-6512 [Electronic] England |
PMID | 26729060
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CD4 Antigens
- CD8 Antigens
- CD8 antigen, alpha chain
- Glial Fibrillary Acidic Protein
- NF-E2-Related Factor 2
- Nfe2l2 protein, mouse
- glial fibrillary astrocytic protein, mouse
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Topics |
- Adult Stem Cells
(physiology)
- Animals
- Apoptosis
- Blood-Brain Barrier
(pathology)
- CD4 Antigens
(metabolism)
- CD8 Antigens
(metabolism)
- Cell Differentiation
- Cell Proliferation
- Encephalomyelitis, Autoimmune, Experimental
(immunology, therapy)
- Endothelium, Vascular
(metabolism, pathology)
- Glial Fibrillary Acidic Protein
(metabolism)
- Male
- Mesenchymal Stem Cell Transplantation
- Mesenchymal Stem Cells
(physiology)
- Mice, Inbred C57BL
- Multiple Sclerosis
(immunology, therapy)
- NF-E2-Related Factor 2
(metabolism)
- Periodontal Ligament
(cytology)
- Primary Cell Culture
- T-Lymphocytes, Regulatory
(immunology)
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