Abstract | OBJECTIVE: METHODS: The method of thiazolyl blue tetrazolium blue (MTT) was employed for determining the proliferation inhibition rate of Chonglou saponin VII plus silica nano composite for SKOV3/DDP drug-resistant human ovarian cancer cell. Flow cytometry and Hoechst 33342 staining were used for detecting apoptosis rate and cell cycle phase distribution. And the changes of apoptosis-related protein were examined by immunoblot assay. RESULTS: There were obvious inhibitory effects of Chonglou saponin VII on cell proliferation. The inhibition rate increased with rising drug concentration with an obvious dose-effect relationship (P < 0.05). At the same time, the cell proliferation inhibition rate significantly improved at 48 h versus 24 h (P < 0.05). Flow cytometry and Hoechst 33342 staining showed that, after a 24 h treatment of SKOV3/DDP cells with 50 and 100 µmol/L Chonglou saponin VII, early apoptotic cell ratio significantly increased compared with control group. CONCLUSION: The composite of Paris polyphylla saponin VII and silica nano has obvious proliferation inhibitory effects on SKOV3/DDP cells. And there is a significant concentration dependence and it also has some correlation with time. The above composite can increased the proportion of early apoptotic cell ratio.
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Authors | Ruiqi Yang, Jie Qi, Jialing Zhang, Fei Wang, Limei Fan |
Journal | Zhonghua yi xue za zhi
(Zhonghua Yi Xue Za Zhi)
Vol. 95
Issue 23
Pg. 1859-61
(Jun 16 2015)
ISSN: 0376-2491 [Print] China |
PMID | 26712407
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Apoptosis
- Cell Line, Tumor
- Cell Proliferation
- Drug Resistance, Neoplasm
- Female
- Humans
- Nanostructures
- Ovarian Neoplasms
- Paris
- Saponins
- Silicon Dioxide
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