The present study was carried out to evaluate the specific and amplified β-
glucuronidase (βG) expression in
prostate cancer cells by using a prostate‑specific
antigen (PSA) promoter-controlled bicistronic adenovirus and to evaluate the specific killing of
prostate cancer cells after the application of the
prodrug DOX‑GA3. Bicistronic adenoviral expression vectors were constructed, and the effectiveness of specific and amplified expression was evaluated using
luciferase and EGFP as reporter genes. βG expression was detected in LNCaP cells after they were infected with the βG‑expressing PSA promoter-controlled bicistronic adenovirus. MTT assays were conducted to evaluate the cytoxicity on the infected cells after the application of the
prodrug DOX‑GA3.
Tumor growth inhibition was also evaluated in nude mice
after treatment with the βG‑expressing adenovirus and DOX‑GA3. Selective and amplified expression was observed in the PSA-producing LNCaP cells, but not in the PSA‑non‑producing DU145 cells. Potent cytotoxity and a strong bystander effect were observed in the LNCaP cells after
infection with the βG‑expressing adenovirus and the application of DOX‑GA3.
Intravenous injection of a GAL4 regulated bicistronic adenovirus vector constructed to express βG under the control of the PSA promoter (Ad/PSAP‑GV16‑βG) and the application of DOX‑GA3 strongly inhibited
tumor growth and prolonged the survival time of tumor‑bearing nude mice. Selective and amplified βG expression together with the
prodrug DOX‑GA3 had an increased antitumor effect, showing great potential for
prostate cancer therapy.