Abstract | BACKGROUND: METHODS: Human bone marrow-derived MSCs (2 × 10(6), passage 5) were administrated intravenously after permanent middle cerebral artery occlusion (MCAO) was induced in male Sprague-Dawley rats. First, we determined the time point of MSC transplantation that led to maximal neurological recovery at 1 h, 1 day, and 3 days after MCAO. Next, we measured activity of MMP-2 and MMP-9, neurological recovery, infarction volume, and vascular density after transplanting MSCs at the time that led to maximal neurological recovery. RESULTS: Among the MSC-transplanted rats, those of the MSC 1-hour group showed maximal recovery in the rotarod test (P = 0.023) and the Longa score (P = 0.018). MMP-2 activity at 1 day after MCAO in the MSC 1-hour group was significantly higher than that in the control group (P = 0.002), but MMP-9 activity was not distinct. The MSC 1-hour group also showed smaller infarction volume and higher vascular density than did the control group. CONCLUSIONS: In a permanent model of rodent MCAO, very early transplantation of human MSCs (1 h after MCAO) produced greater neurological recovery and decreased infraction volume. The elevation of MMP-2 activity and the increase in vascular density after MSC treatment suggest that MSCs might help promote angiogenesis and lead to neurological improvement during the recovery phase after ischemic stroke.
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Authors | Hyo Suk Nam, Il Kwon, Bo Hyung Lee, Haejin Kim, Jayoung Kim, Sunho An, Ok-Hee Lee, Phil Hyu Lee, Hyun Ok Kim, Hyun Namgoong, Young Dae Kim, Ji Hoe Heo |
Journal | PloS one
(PLoS One)
Vol. 10
Issue 12
Pg. e0144218
( 2015)
ISSN: 1932-6203 [Electronic] United States |
PMID | 26637168
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Matrix Metalloproteinases
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Topics |
- Animals
- Brain Infarction
(metabolism, physiopathology, therapy)
- Cells, Cultured
- Disease Models, Animal
- Humans
- Male
- Matrix Metalloproteinases
(metabolism)
- Mesenchymal Stem Cell Transplantation
(methods)
- Neovascularization, Pathologic
(metabolism, therapy)
- Rats
- Rats, Sprague-Dawley
- Rotarod Performance Test
- Stroke
(metabolism, physiopathology, therapy)
- Treatment Outcome
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