Polydatin Induces Apoptosis and Inhibits Growth of Acute Monocytic Leukemia Cells.

Abstract

Polydatin (PD), a component isolated from Polygonum cuspidatum, has various activities such as inhibiting platelet aggregation, lowering level of blood lipid, reducing lipid peroxidation, and so on. However, the antitumor activity of PD has been poorly reported. In the present study, effect of PD on cell proliferation was evaluated by Cell Counting Kit-8, and cell cycle and apoptosis were investigated by flow cytometry. Meanwhile, the protein expression level of Bc1-2, Bax, cyclin A, cyclin B, and cyclin D1, which associated with apoptosis and cell cycle were analyzed by Western blotting. Results show that PD could effectively inhibit the growth, arrest cells in S phase, and induce apoptosis of acute monocytic leukemia cell line THP-1; meanwhile, expression of cyclin D1 and Bc1-2 decreased significantly, and expression of Bax and cyclin A increased notably. All results suggest that PD maybe a potential therapeutic strategy for acute monocytic leukemia.

• Authors: Chunmei Wang, Yuan Luo, Jie Lu, Yingchao Wang, Guangyao Sheng
• Journal: Journal of biochemical and molecular toxicology (J Biochem Mol Toxicol) Vol. 30 Issue 4 Pg. 200-5 (Apr 2016) ISSN: 1099-0461 [Electronic] United States
• PMID: 26616494 (Publication Type: Journal Article)
• Copyright: © 2015 Wiley Periodicals, Inc.

Chemical References

• BAX protein, human
• Cyclins
• Glucosides
• Proto-Oncogene Proteins c-bcl-2
• Stilbenes
• bcl-2-Associated X Protein
• polydatin

Topics

• Apoptosis (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cyclins (metabolism)
• Glucosides (pharmacology)
• Humans
• Leukemia, Monocytic, Acute (metabolism, pathology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• S Phase (drug effects)
• Stilbenes (pharmacology)
• bcl-2-Associated X Protein (metabolism)