Alterations of Bio-elements, Oxidative, and Inflammatory Status in the Zinc Deficiency Model in Rats.

Abstract

Our previous study showed that dietary zinc restriction induces depression-like behavior with concomitant up-regulation of the N-methyl-D-aspartate receptor (NMDAR). Because metal ions, oxidative stress, and inflammation are involved in depression/NMDAR function, in the present study, bio-elements (zinc, copper, iron, magnesium, and calcium), oxidative (thiobarbituric acid-reactive substances; protein carbonyl content), and inflammatory (IL-1α, IL-1β) factors were measured in serum, hippocampus (Hp), and prefrontal cortex (PFC) of male Sprague-Dawley rats subjected to a zinc-adequate (ZnA) (50 mg Zn/kg) or a zinc-deficient (ZnD) (3 mg Zn/kg) diet for 4 or 6 weeks. Both periods of dietary zinc restriction reduced serum zinc and increased serum iron levels. At 4 weeks, lowered zinc level in the PFC and Hp as well as lowered iron level in the PFC of the ZnD rats was observed. At 6 weeks, however, iron level was increased in the PFC of these rats. Although at 6 weeks zinc level in the PFC did not differ between the ZnA and ZnD rats, extracellular zinc concentration after 100 mM KCl stimulation was reduced in the PFC of the ZnD rats and was accompanied by increased extracellular iron and glutamate levels (as measured by the in vivo microdialysis). The examined oxidative and inflammatory parameters were generally enhanced in the tissue of the ZnD animals. The obtained data suggest dynamic redistribution of bio-elements and enhancement of oxidative/inflammatory parameters after dietary zinc restriction, which may have a link with depression-like behavior/NMDAR function/neurodegeneration.

• Authors: Urszula Doboszewska, Bernadeta Szewczyk, Magdalena Sowa-Kućma, Karolina Noworyta-Sokołowska, Paulina Misztak, Joanna Gołębiowska, Katarzyna Młyniec, Beata Ostachowicz, Mirosław Krośniak, Agnieszka Wojtanowska-Krośniak, Krystyna Gołembiowska, Marek Lankosz, Wojciech Piekoszewski, Gabriel Nowak
• Journal: Neurotoxicity research (Neurotox Res) Vol. 29 Issue 1 Pg. 143-54 (Jan 2016) ISSN: 1476-3524 [Electronic] United States
• PMID: 26581375 (Publication Type: Journal Article)

Chemical References

• Interleukin-1alpha
• Interleukin-1beta
• Thiobarbituric Acid Reactive Substances
• Potassium Chloride
• Copper
• Iron
• Zinc
• Corticosterone

Topics

• Animals
• Body Weight
• Copper (metabolism)
• Corticosterone (metabolism)
• Diet (adverse effects)
• Dose-Response Relationship, Drug
• Hippocampus (metabolism)
• Interleukin-1alpha (blood)
• Interleukin-1beta (blood)
• Iron (metabolism)
• Male
• Oxidative Stress (drug effects)
• Potassium Chloride (pharmacology)
• Prefrontal Cortex (drug effects, metabolism)
• Protein Carbonylation (drug effects)
• Rats
• Rats, Sprague-Dawley
• Thiobarbituric Acid Reactive Substances (metabolism)
• Time Factors
• Zinc (administration & dosage, blood, deficiency)