Myocardial expression profiles of candidate molecules in patients with arrhythmogenic right ventricular cardiomyopathy/dysplasia compared to those with dilated cardiomyopathy and healthy controls.

Abstract

BACKGROUND:

Arrhythmogenic right ventricular cardiomyopathy/dysplasia (ARVC/D) is mainly an autosomal dominant disease characterized by fibrofatty infiltration of the right ventricle, leading to ventricular arrhythmias. Mutations in desmosomal proteins can be identified in about half of the patients. The pathogenic mechanisms leading to disease expression remain unclear.

OBJECTIVE:

The purpose of this study was to investigate myocardial expression profiles of candidate molecules involved in the pathogenesis of ARVC/D.

METHODS:

Myocardial messenger RNA (mRNA) expression of 62 junctional molecules, 5 cardiac ion channel molecules, 8 structural molecules, 4 apoptotic molecules, and 6 adipogenic molecules was studied. The averaged expression of candidate mRNAs was compared between ARVC/D samples (n = 10), nonfamilial dilated cardiomyopathy (DCM) samples (n = 10), and healthy control samples (n = 8). Immunohistochemistry and quantitative protein expression analysis were performed. Genetic analysis using next generation sequencing was performed in all patients with ARVC/D.

RESULTS:

Following mRNA levels were significantly increased in patients with ARVC/D compared to those with DCM and healthy controls: phospholamban (P ≤ .001 vs DCM; P ≤ .001 vs controls), healthy tumor protein 53 apoptosis effector (P = .001 vs DCM; P ≤ .001 vs controls), and carnitine palmitoyltransferase 1β (P ≤ .001 vs DCM; P = 0.008 vs controls). Plakophillin-2 (PKP-2) mRNA was downregulated in patients with ARVC/D with PKP-2 mutations compared with patients with ARVC/D without PKP-2 mutations (P = .04). Immunohistochemistry revealed significantly increased protein expression of phospholamban, tumor protein 53 apoptosis effector, and carnitine palmitoyltransferase 1β in patients with ARVC/D and decreased PKP-2 expression in patients with ARVC/D carrying a PKP-2 mutation.

CONCLUSION:

Changes in the expression profiles of sarcolemmal calcium channel regulation, apoptosis, and adipogenesis suggest that these molecular pathways may play a critical role in the pathogenesis of ARVC/D, independent of the underlying genetic mutations.

• Authors: Deniz Akdis, Argelia Medeiros-Domingo, Anna Gaertner-Rommel, Jeannette I Kast, Frank Enseleit, Peter Bode, Karin Klingel, Reinhard Kandolf, Fanny Renois, Laurent Andreoletti, Cezmi A Akdis, Hendrik Milting, Thomas F Lüscher, Corinna Brunckhorst, Ardan M Saguner, Firat Duru
• Journal: Heart rhythm (Heart Rhythm) Vol. 13 Issue 3 Pg. 731-41 (Mar 2016) ISSN: 1556-3871 [Electronic] United States
• PMID: 26569459 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers
• RNA, Messenger

Topics

• Apoptosis (genetics)
• Arrhythmogenic Right Ventricular Dysplasia (genetics, metabolism, physiopathology)
• Biomarkers (metabolism)
• Cardiomyopathy, Dilated (genetics, metabolism, physiopathology)
• Female
• Gene Expression Regulation
• Genetic Association Studies (methods)
• Genetic Testing
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• Myocardium (metabolism)
• RNA, Messenger (genetics)