Preparation of quadri-subtype influenza virus-like particles using bovine immunodeficiency virus gag protein.

Abstract

Influenza VLPs comprised of hemagglutinin (HA), neuraminidase (NA), and matrix (M1) proteins have been previously used for immunological and virological studies. Here we demonstrated that influenza VLPs can be made in Sf9 cells by using the bovine immunodeficiency virus gag (Bgag) protein in place of M1. We showed that Bgag can be used to prepare VLPs for several influenza subtypes including H1N1 and H10N8. Furthermore, by using Bgag, we prepared quadri-subtype VLPs, which co-expressed within the VLP the four HA subtypes derived from avian-origin H5N1, H7N9, H9N2 and H10N8 viruses. VLPs showed hemagglutination and neuraminidase activities and reacted with specific antisera. The content and co-localization of each HA subtype within the quadri-subtype VLP were evaluated. Electron microscopy showed that Bgag-based VLPs resembled influenza virions with the diameter of 150-200nm. This is the first report of quadri-subtype design for influenza VLP and the use of Bgag for influenza VLP preparation.

• Authors: Irina Tretyakova, Rachmat Hidajat, Garrett Hamilton, Noah Horn, Brian Nickols, Raphael O Prather, Terrence M Tumpey, Peter Pushko
• Journal: Virology (Virology) Vol. 487 Pg. 163-71 (Jan 2016) ISSN: 1096-0341 [Electronic] United States
• PMID: 26529299 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Antibodies, Viral
• Gene Products, gag
• Hemagglutinin Glycoproteins, Influenza Virus
• Vaccines, Virus-Like Particle
• Neuraminidase

Topics

• Animals
• Antibodies, Viral (immunology)
• Cell Line
• Gene Products, gag (genetics, immunology)
• Hemagglutinin Glycoproteins, Influenza Virus (immunology)
• Immunodeficiency Virus, Bovine (genetics, immunology)
• Influenza A Virus, H10N8 Subtype (genetics, immunology)
• Influenza A Virus, H1N1 Subtype (genetics, immunology)
• Influenza A Virus, H5N1 Subtype (genetics, immunology)
• Influenza A Virus, H7N9 Subtype (genetics, immunology)
• Influenza A Virus, H9N2 Subtype (genetics, immunology)
• Insecta
• Neuraminidase (immunology)
• Orthomyxoviridae Infections (immunology, prevention & control, virology)
• Sf9 Cells
• Spodoptera
• Vaccines, Virus-Like Particle (immunology)