Abstract |
Yeast phenylalanine ammonia-lyase was administered i.p. to normal and tumor-bearing mice, and its clearance from plasma was studied. Single and multiple weekly injections at dosages of 10,20,50 and 100 units/kg were administered to C57BL female, C57BL X DBA/2F1 male, and A/J female mice. L5178Y murine lymphoblastic leukemia, B16 melanoma, BW10232 adenocarcinoma, and 15091A anaplastic carcinoma were implanted 7 to 11 days prior to enzyme injection in the appropriate host. After a single injection, the average plasma half-lives of phenylalanine ammonia-lyase were 18 to 24 hr in all groups studied. While the other tumors had no effect on the plasma level of phenylalanine ammonia-lyase after a single injection, L5178Y murine lymphoblastic leukemia and 15091A anaplastic carcinoma significantly depressed the maximal level of phenylalanine ammonia-lyase attained in the plasma. After repeated injections of phenylalanine ammonia-lyase, the initial plasma enzyme level was significantly reduced when 20 units/kg were administered, and the clearance of the enzyme from the plasma was greatly accelerated regardless of the amount administered. Furthermore, in tumor-bearing mice, the rate of clearance was significantly more rapid than in the appropriate non- tumor-bearing control.
|
Authors | R S Shen, R R Fritz, C W Abell |
Journal | Cancer research
(Cancer Res)
Vol. 37
Issue 4
Pg. 1051-6
(Apr 1977)
ISSN: 0008-5472 [Print] United States |
PMID | 265185
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Antigens
- Ammonia-Lyases
- Phenylalanine Ammonia-Lyase
|
Topics |
- Adenocarcinoma
(blood)
- Ammonia-Lyases
(blood)
- Animals
- Antibody Formation
- Antigens
- Carcinoma
(blood)
- Dose-Response Relationship, Drug
- Drug Administration Schedule
- Female
- Half-Life
- Injections, Intraperitoneal
- Leukemia, Lymphoid
(blood)
- Male
- Melanoma
(blood)
- Metabolic Clearance Rate
- Mice
- Mice, Inbred A
- Mice, Inbred C57BL
- Mice, Inbred DBA
- Neoplasms, Experimental
(blood, immunology)
- Phenylalanine Ammonia-Lyase
(administration & dosage, blood, immunology)
|